Laser capture microdissection in molecular diagnostics

Liang Cheng, Shaobo Zhang, Darrell D. Davidson, Matthew Kuhar, Mingsheng Wang, Sean R. Williamson, David Y. Zhang, Gregory T. MacLennan

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The myriad of cell types within neoplasms presents a major obstacle for the global analysis of such tissues. Laser capture microdissection employs a laser to dissect individual cells or small clusters of cells selected by concurrent light microscopy. Laser microdissection permits analysis of molecular alterations within specific cell populations without interference from nontarget cell populations. Laser capture microdissection has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. Currently there are two major technical platforms: the infrared laser - thermal-sensitive membrane system - and the foil-cutting ultraviolet laser system.

Original languageEnglish (US)
Title of host publicationMolecular Genetic Pathology
Subtitle of host publicationSecond Edition
PublisherSpringer New York
Pages465-482
Number of pages18
ISBN (Electronic)9781461448006
ISBN (Print)1461447992, 9781461447993
DOIs
StatePublished - Mar 1 2014

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cheng, L., Zhang, S., Davidson, D. D., Kuhar, M., Wang, M., Williamson, S. R., Zhang, D. Y., & MacLennan, G. T. (2014). Laser capture microdissection in molecular diagnostics. In Molecular Genetic Pathology: Second Edition (pp. 465-482). Springer New York. https://doi.org/10.1007/978-1-4614-4800-6-16