Laser capture microdissection in molecular diagnostics

Liang Cheng, Shaobo Zhang, Darrell Davidson, Matthew Kuhar, Mingsheng Wang, Sean R. Williamson, David Y. Zhang, Gregory T. MacLennan

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The myriad of cell types within neoplasms presents a major obstacle for the global analysis of such tissues. Laser capture microdissection employs a laser to dissect individual cells or small clusters of cells selected by concurrent light microscopy. Laser microdissection permits analysis of molecular alterations within specific cell populations without interference from nontarget cell populations. Laser capture microdissection has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. Currently there are two major technical platforms: the infrared laser - thermal-sensitive membrane system - and the foil-cutting ultraviolet laser system.

Original languageEnglish
Title of host publicationMolecular Genetic Pathology: Second Edition
PublisherSpringer New York
Pages465-482
Number of pages18
ISBN (Print)9781461448006, 1461447992, 9781461447993
DOIs
StatePublished - Mar 1 2014

Fingerprint

Laser Capture Microdissection
Molecular Pathology
Lasers
Neoplasms
Microdissection
Metabolomics
Gene Expression Profiling
Epigenomics
Proteomics
Population
Microscopy
Hot Temperature
Light
Mutation
Membranes
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cheng, L., Zhang, S., Davidson, D., Kuhar, M., Wang, M., Williamson, S. R., ... MacLennan, G. T. (2014). Laser capture microdissection in molecular diagnostics. In Molecular Genetic Pathology: Second Edition (pp. 465-482). Springer New York. https://doi.org/10.1007/978-1-4614-4800-6-16

Laser capture microdissection in molecular diagnostics. / Cheng, Liang; Zhang, Shaobo; Davidson, Darrell; Kuhar, Matthew; Wang, Mingsheng; Williamson, Sean R.; Zhang, David Y.; MacLennan, Gregory T.

Molecular Genetic Pathology: Second Edition. Springer New York, 2014. p. 465-482.

Research output: Chapter in Book/Report/Conference proceedingChapter

Cheng, L, Zhang, S, Davidson, D, Kuhar, M, Wang, M, Williamson, SR, Zhang, DY & MacLennan, GT 2014, Laser capture microdissection in molecular diagnostics. in Molecular Genetic Pathology: Second Edition. Springer New York, pp. 465-482. https://doi.org/10.1007/978-1-4614-4800-6-16
Cheng L, Zhang S, Davidson D, Kuhar M, Wang M, Williamson SR et al. Laser capture microdissection in molecular diagnostics. In Molecular Genetic Pathology: Second Edition. Springer New York. 2014. p. 465-482 https://doi.org/10.1007/978-1-4614-4800-6-16
Cheng, Liang ; Zhang, Shaobo ; Davidson, Darrell ; Kuhar, Matthew ; Wang, Mingsheng ; Williamson, Sean R. ; Zhang, David Y. ; MacLennan, Gregory T. / Laser capture microdissection in molecular diagnostics. Molecular Genetic Pathology: Second Edition. Springer New York, 2014. pp. 465-482
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