Laser-induced noninvasive vascular injury models in mice generate platelet- and coagulationdependent thrombi

Elliot D. Rosen, Sylvain Raymond, Amy Zollman, Francisco Noria, Mayra Sandoval-Cooper, Alexis Shulman, James L. Merz, Francis J. Castellino

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

A minimally invasive laser-induced injury model is described to study thrombus development in mice in vivo. The protocol involves focusing the beam of an argon-ion laser through a compound microscope on the vasculature of a mouse ear that is sufficiently thin such that blood flow can be visualized by intravital microscopy. Two distinct injury models have been established. The first involves direct laser illumination with a short, high-intensity pulse. In this case, thrombus formation is inhibited by the GPIIb/IIIa antagonist, G4120. However, the anticoagulants, hirulog, PPACK, and NapC2 have minimal effect. This indicates that thrombus development induced by this model mainly involves platelet interactions. The second model involves low-intensity laser illumination of mice injected with Rose Bengal dye to induce photochemical injury in the region of laser illumination. Thrombi generated by this latter procedure have a slower development and are inhibited by both anti-coagulant and anti-platelet compounds.

Original languageEnglish (US)
Pages (from-to)1613-1622
Number of pages10
JournalAmerican Journal of Pathology
Volume158
Issue number5
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Rosen, E. D., Raymond, S., Zollman, A., Noria, F., Sandoval-Cooper, M., Shulman, A., Merz, J. L., & Castellino, F. J. (2001). Laser-induced noninvasive vascular injury models in mice generate platelet- and coagulationdependent thrombi. American Journal of Pathology, 158(5), 1613-1622. https://doi.org/10.1016/S0002-9440(10)64117-X