Laser raman studies of lipid disordering by the B-protein of fd phage

A. Dunker, Robert W. Williams, Bruce P. Gaber, Warner L. Peticolas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Complexes of the B-protein of fd phage with the model lipid dipalmitoyl phosphatidylcholine (DPPC) were made by sonication of the fd phage in the presence of dipalmitoyl phosphatidylcholine. Both laser Raman spectra and circular dichroism show the protein in the membrane to be almost entirely in the β-sheet conformation. This β-sheet conformation is found to be independent of the temperature between 10° C and 50° C. On the other hand, the protein has a very dramatic effect on the organization of the lipid bilayer. An aqueous dispersion of 1 : 1 lipid/protein mixture gives a broad conformational transition of DPPC which occurs between 10° C and 30° C. This contrasts markedly with simple aqueous DPPC dispersions which show a sharp transition at 41°C. This appears to be the first reported example of the lowering of the conformational transition of a membrane bilayer by an intrinsic membrane protein.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume553
Issue number2
DOIs
StatePublished - May 17 1979
Externally publishedYes

Fingerprint

Bacteriophage M13
1,2-Dipalmitoylphosphatidylcholine
Bacteriophages
Lasers
Lipids
Conformations
Membrane Proteins
Membranes
Proteins
Sonication
Lipid bilayers
Lipid Bilayers
Circular Dichroism
Dispersions
Raman scattering
Temperature
IgA receptor

Keywords

  • (fd phage)
  • B protein
  • Laser Raman
  • Lipid disordering

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Laser raman studies of lipid disordering by the B-protein of fd phage. / Dunker, A.; Williams, Robert W.; Gaber, Bruce P.; Peticolas, Warner L.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 553, No. 2, 17.05.1979, p. 351-357.

Research output: Contribution to journalArticle

Dunker, A. ; Williams, Robert W. ; Gaber, Bruce P. ; Peticolas, Warner L. / Laser raman studies of lipid disordering by the B-protein of fd phage. In: Biochimica et Biophysica Acta - Biomembranes. 1979 ; Vol. 553, No. 2. pp. 351-357.
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