Late gestational vascular disruptions inducing craniofacial anomalies: A fetal lamb model

Luis F. Escobar, Edward A. Liechty

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We report our preliminary observations in six fetal lambs that were surgically manipulated in utero to impede the blood flow of the carotid arteries and their branches, including the laryngeal artery, the anastomotic branch between the vertebral artery and the occipital artery, the auricularis and the transverse facial arteries. Between 115 and 117 days of gestation (term pregnancy 147 days), all ewes were placed under general anesthesia and mechanical ventilation. Their fetuses were exteriorized and catheters were placed in their femoral artery for blood gas sampling. A balloon occluder and a blood flow probe were placed on one internal carotid while the contralateral side was completely ligated. On the third day post surgery, the balloon occluder was inflated three times for 30 minutes each time at 30 minute intervals in the experimental fetuses. PO2, PCO2, pH, lactate and glucose were monitored during the study. At 7 days post occlusion, all animals were sacrificed and tissues were collected. Craniofacial anomalies were obvious in three animals similar to those seen in hemifacial microsomia, Goldenhar syndrome and Pierre-Robin sequence. All three control animals had normal craniofacial structures. This preliminary data suggests that late gestation vascular disruptions may lead to significant craniofacial anomalies, as seen in our animal model.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalJournal of Craniofacial Genetics and Developmental Biology
Volume18
Issue number3
StatePublished - 1998

Fingerprint

Goldenhar Syndrome
Blood Vessels
Arteries
Pregnancy
Fetus
Pierre Robin Syndrome
Vertebral Artery
Femoral Artery
Ambulatory Surgical Procedures
Carotid Arteries
Artificial Respiration
General Anesthesia
Ear
Lactic Acid
Catheters
Animal Models
Gases
Glucose

Keywords

  • Craniofacial anomalies
  • Hemifacial microsomia
  • Micrognathia
  • Pierre-Robin sequence
  • Vascular disruptions

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Late gestational vascular disruptions inducing craniofacial anomalies : A fetal lamb model. / Escobar, Luis F.; Liechty, Edward A.

In: Journal of Craniofacial Genetics and Developmental Biology, Vol. 18, No. 3, 1998, p. 159-163.

Research output: Contribution to journalArticle

@article{6c6c898ee05941b79a5a00054f8fcbe2,
title = "Late gestational vascular disruptions inducing craniofacial anomalies: A fetal lamb model",
abstract = "We report our preliminary observations in six fetal lambs that were surgically manipulated in utero to impede the blood flow of the carotid arteries and their branches, including the laryngeal artery, the anastomotic branch between the vertebral artery and the occipital artery, the auricularis and the transverse facial arteries. Between 115 and 117 days of gestation (term pregnancy 147 days), all ewes were placed under general anesthesia and mechanical ventilation. Their fetuses were exteriorized and catheters were placed in their femoral artery for blood gas sampling. A balloon occluder and a blood flow probe were placed on one internal carotid while the contralateral side was completely ligated. On the third day post surgery, the balloon occluder was inflated three times for 30 minutes each time at 30 minute intervals in the experimental fetuses. PO2, PCO2, pH, lactate and glucose were monitored during the study. At 7 days post occlusion, all animals were sacrificed and tissues were collected. Craniofacial anomalies were obvious in three animals similar to those seen in hemifacial microsomia, Goldenhar syndrome and Pierre-Robin sequence. All three control animals had normal craniofacial structures. This preliminary data suggests that late gestation vascular disruptions may lead to significant craniofacial anomalies, as seen in our animal model.",
keywords = "Craniofacial anomalies, Hemifacial microsomia, Micrognathia, Pierre-Robin sequence, Vascular disruptions",
author = "Escobar, {Luis F.} and Liechty, {Edward A.}",
year = "1998",
language = "English",
volume = "18",
pages = "159--163",
journal = "Journal of Craniofacial Genetics and Developmental Biology",
issn = "0270-4145",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Late gestational vascular disruptions inducing craniofacial anomalies

T2 - A fetal lamb model

AU - Escobar, Luis F.

AU - Liechty, Edward A.

PY - 1998

Y1 - 1998

N2 - We report our preliminary observations in six fetal lambs that were surgically manipulated in utero to impede the blood flow of the carotid arteries and their branches, including the laryngeal artery, the anastomotic branch between the vertebral artery and the occipital artery, the auricularis and the transverse facial arteries. Between 115 and 117 days of gestation (term pregnancy 147 days), all ewes were placed under general anesthesia and mechanical ventilation. Their fetuses were exteriorized and catheters were placed in their femoral artery for blood gas sampling. A balloon occluder and a blood flow probe were placed on one internal carotid while the contralateral side was completely ligated. On the third day post surgery, the balloon occluder was inflated three times for 30 minutes each time at 30 minute intervals in the experimental fetuses. PO2, PCO2, pH, lactate and glucose were monitored during the study. At 7 days post occlusion, all animals were sacrificed and tissues were collected. Craniofacial anomalies were obvious in three animals similar to those seen in hemifacial microsomia, Goldenhar syndrome and Pierre-Robin sequence. All three control animals had normal craniofacial structures. This preliminary data suggests that late gestation vascular disruptions may lead to significant craniofacial anomalies, as seen in our animal model.

AB - We report our preliminary observations in six fetal lambs that were surgically manipulated in utero to impede the blood flow of the carotid arteries and their branches, including the laryngeal artery, the anastomotic branch between the vertebral artery and the occipital artery, the auricularis and the transverse facial arteries. Between 115 and 117 days of gestation (term pregnancy 147 days), all ewes were placed under general anesthesia and mechanical ventilation. Their fetuses were exteriorized and catheters were placed in their femoral artery for blood gas sampling. A balloon occluder and a blood flow probe were placed on one internal carotid while the contralateral side was completely ligated. On the third day post surgery, the balloon occluder was inflated three times for 30 minutes each time at 30 minute intervals in the experimental fetuses. PO2, PCO2, pH, lactate and glucose were monitored during the study. At 7 days post occlusion, all animals were sacrificed and tissues were collected. Craniofacial anomalies were obvious in three animals similar to those seen in hemifacial microsomia, Goldenhar syndrome and Pierre-Robin sequence. All three control animals had normal craniofacial structures. This preliminary data suggests that late gestation vascular disruptions may lead to significant craniofacial anomalies, as seen in our animal model.

KW - Craniofacial anomalies

KW - Hemifacial microsomia

KW - Micrognathia

KW - Pierre-Robin sequence

KW - Vascular disruptions

UR - http://www.scopus.com/inward/record.url?scp=0031687994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031687994&partnerID=8YFLogxK

M3 - Article

C2 - 9785220

AN - SCOPUS:0031687994

VL - 18

SP - 159

EP - 163

JO - Journal of Craniofacial Genetics and Developmental Biology

JF - Journal of Craniofacial Genetics and Developmental Biology

SN - 0270-4145

IS - 3

ER -