Latent forms of transforming growth factor-β (TGFβ) derived from bone cultures

Identification of a naturally occurring 100-kDa complex with similarity to recombinant latent TGFβ

Lynda Bonewald, Lalage Wakefield, R. O C Oreffo, Alda Escobedo, Daniel R. Twardzik, Gregory R. Mundy

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Transforming growth factor-β (TGFβ) is produced by most tissues, including bone, as a complex that is biologically inert. Release of TGFβ homodimer from this latent complex is necessary for TGFβ to exert effects on target cells. Thus, the nature of the latent complex and the mechanisms responsible for TGFβ release are the key to understanding TGFβ actions. We have found that murine calvarial bone cultures secrete multiple latent forms of TGFβ. Using analytical chromatography and Western blot analysis, we have compared bone latent TGFβ with the previously characterized latent complex present in platelets and with simian TGFβ precursor, which is stably expressed in a latent form by Chinese hamster ovarian (CHO) cells. A major component of the bone material appears to be a latent complex of 100 kDa, consisting of mature TGFβ (25-kDa homodimer) non-covalently associated with the remainder of the TGFβ precursor proregion (75-kDa homodimer). Like the recombinant TGFβ precursor, it elutes from a Mono-Q fast pressure liquid chromatography anion exchange column at 0.2 M NaCl and shows a very similar banding pattern on Western blots. Thus, this bone complex closely resembles recombinant TGFβ precursor expressed in a latent form by CHO cells and differs from the naturally occurring platelet complex, which has an additional 135-kDa binding protein that is bound through disulfide bonds to the precursor proregion. Western blot analysis also indicates that, like CHO cells, which express recombinant TGFβ precursor, but unlike other cell types, the bone cultures secrete detectable amounts of uncleaved TGFβ precursor. The bone calvarial culture is the first example of a naturally occurring system that expresses the 100-kDa latent TGFβ complex.

Original languageEnglish (US)
Pages (from-to)741-751
Number of pages11
JournalMolecular Endocrinology
Volume5
Issue number6
StatePublished - Jun 1991
Externally publishedYes

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Transforming Growth Factors
Transforming Growth Factor beta
Bone and Bones
Cricetulus
Western Blotting
Blood Platelets
Liquid Chromatography
Disulfides
Anions
Chromatography
Carrier Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Latent forms of transforming growth factor-β (TGFβ) derived from bone cultures : Identification of a naturally occurring 100-kDa complex with similarity to recombinant latent TGFβ. / Bonewald, Lynda; Wakefield, Lalage; Oreffo, R. O C; Escobedo, Alda; Twardzik, Daniel R.; Mundy, Gregory R.

In: Molecular Endocrinology, Vol. 5, No. 6, 06.1991, p. 741-751.

Research output: Contribution to journalArticle

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