Lean and Obese Coronary Perivascular Adipose Tissue Impairs Vasodilation via Differential Inhibition of Vascular Smooth Muscle K<sup>+</sup> Channels

Jillian N. Noblet, Meredith K. Owen, Adam G. Goodwill, Daniel J. Sassoon, Johnathan Tune

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective - The effects of coronary perivascular adipose tissue (PVAT) on vasomotor tone are influenced by an obese phenotype and are distinct from other adipose tissue depots. The purpose of this investigation was to examine the effects of lean and obese coronary PVAT on end-effector mechanisms of coronary vasodilation and to identify potential factors involved. Approach and Results - Hematoxylin and eosin staining revealed similarities in coronary perivascular adipocyte size between lean and obese Ossabaw swine. Isometric tension studies of isolated coronary arteries from Ossabaw swine revealed that factors derived from lean and obese coronary PVAT attenuated vasodilation to adenosine. Lean coronary PVAT inhibited K<inf>Ca</inf>and K<inf>V</inf>7, but not K<inf>ATP</inf>channel-mediated dilation in lean arteries. In the absence of PVAT, vasodilation to K<inf>Ca</inf>and K<inf>V</inf>7 channel activation was impaired in obese arteries relative to lean arteries. Obese PVAT had no effect on K<inf>Ca</inf>or K<inf>V</inf>7 channel-mediated dilation in obese arteries. In contrast, obese PVAT inhibited K<inf>ATP</inf>channel-mediated dilation in both lean and obese arteries. The differential effects of obese versus lean PVAT were not associated with changes in either coronary K<inf>V</inf>7 or K<inf>ATP</inf>channel expression. Incubation with calpastatin attenuated coronary vasodilation to adenosine in lean but not in obese arteries. Conclusions - These findings indicate that lean and obese coronary PVAT attenuates vasodilation via inhibitory effects on vascular smooth muscle K<sup>+</sup> channels and that alterations in specific factors such as calpastatin are capable of contributing to the initiation or progression of smooth muscle dysfunction in obesity.

Original languageEnglish
Pages (from-to)1393-1400
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume35
Issue number6
DOIs
StatePublished - Jun 27 2015

Fingerprint

Vascular Smooth Muscle
Vasodilation
Adipose Tissue
Arteries
Dilatation
Adenosine
Swine
Hematoxylin
Eosine Yellowish-(YS)
Adipocytes
Smooth Muscle
Coronary Vessels
Obesity
Staining and Labeling
Phenotype

Keywords

  • adipose tissue
  • coronary
  • obesity
  • vasodilation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Lean and Obese Coronary Perivascular Adipose Tissue Impairs Vasodilation via Differential Inhibition of Vascular Smooth Muscle K<sup>+</sup> Channels. / Noblet, Jillian N.; Owen, Meredith K.; Goodwill, Adam G.; Sassoon, Daniel J.; Tune, Johnathan.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 35, No. 6, 27.06.2015, p. 1393-1400.

Research output: Contribution to journalArticle

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N2 - Objective - The effects of coronary perivascular adipose tissue (PVAT) on vasomotor tone are influenced by an obese phenotype and are distinct from other adipose tissue depots. The purpose of this investigation was to examine the effects of lean and obese coronary PVAT on end-effector mechanisms of coronary vasodilation and to identify potential factors involved. Approach and Results - Hematoxylin and eosin staining revealed similarities in coronary perivascular adipocyte size between lean and obese Ossabaw swine. Isometric tension studies of isolated coronary arteries from Ossabaw swine revealed that factors derived from lean and obese coronary PVAT attenuated vasodilation to adenosine. Lean coronary PVAT inhibited KCaand KV7, but not KATPchannel-mediated dilation in lean arteries. In the absence of PVAT, vasodilation to KCaand KV7 channel activation was impaired in obese arteries relative to lean arteries. Obese PVAT had no effect on KCaor KV7 channel-mediated dilation in obese arteries. In contrast, obese PVAT inhibited KATPchannel-mediated dilation in both lean and obese arteries. The differential effects of obese versus lean PVAT were not associated with changes in either coronary KV7 or KATPchannel expression. Incubation with calpastatin attenuated coronary vasodilation to adenosine in lean but not in obese arteries. Conclusions - These findings indicate that lean and obese coronary PVAT attenuates vasodilation via inhibitory effects on vascular smooth muscle K+ channels and that alterations in specific factors such as calpastatin are capable of contributing to the initiation or progression of smooth muscle dysfunction in obesity.

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