Legionella indianapolisensis sp. nov., isolated from a patient with pulmonary abscess

Ryan Relich, Bryan Schmitt, H. Raposo, L. Barker, S. J. Blosser, M. May

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: To date, at least 50 species of Legionella have been described. These organisms are ubiquitous in nature and have been isolated from diverse ecological environments, including man-made structures such as cooling towers and spas. Legionellae have also been isolated from human and veterinary clinical specimens, and their roles in disease are well-established. This report describes the isolation of a novel Legionella species from a respiratory specimen from a patient with influenza and suspected pulmonary embolus. Case: A 68-year-old male presented to an Indianapolis-area hospital with pulmonary disease; upon workup, he was found to have influenza A. Bronchoalveolar lavage fluid was also submitted for conventional bacterial culture and Legionella culture. The patient was prescribed a broad-spectrum antibiotic and recovered. Results: A Legionella-like bacterium was isolated on buffered charcoal yeast extract agar, and mass spectrometry and comparative 16S rRNA gene sequencing inconclusively identified the isolate as a Legionella sp. Further analysis of the 16S rRNA gene confirmed the strain to be a new species, related to Legionella hackeliae. Physiochemical and morphological testing were used to confirm the discovery of a novel species, Legionella indianapolisensis sp. nov., type strain SMNF-IS.

Original languageEnglish (US)
Pages (from-to)26-28
Number of pages3
JournalInternational Journal of Infectious Diseases
Volume69
DOIs
StatePublished - Apr 1 2018

Fingerprint

Lung Abscess
Legionella
rRNA Genes
Human Influenza
Charcoal
Bronchoalveolar Lavage Fluid
Embolism
Lung Diseases
Agar
Mass Spectrometry
Yeasts
Anti-Bacterial Agents
Bacteria
Lung

Keywords

  • Co-infection
  • Influenza
  • Legionella
  • Legionella indianapolisensis
  • Legionellae
  • Lobar pneumonia
  • Pulmonary abscess

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Legionella indianapolisensis sp. nov., isolated from a patient with pulmonary abscess. / Relich, Ryan; Schmitt, Bryan; Raposo, H.; Barker, L.; Blosser, S. J.; May, M.

In: International Journal of Infectious Diseases, Vol. 69, 01.04.2018, p. 26-28.

Research output: Contribution to journalArticle

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