Lens epithelial cell proliferation, migration, and metaplasia following capsulorhexis

Lisa Saxby, Emanuel Rosen, Mike Boulton

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Aim - To study the behaviour of residual lens epithelial cells after capsulorhexis and expression of material from the isolated lens. Methods - Human and bovine lens capsules were isolated, sterile non-toxic silicone rings inserted, and the preparations placed in organ culture for up to 12 weeks. Cell coverage of the posterior lens capsule was recorded and the capsules were examined, both pre- and post-coverage, for (a) proliferative activity and (b) cytoskeletal components. Results - After a lag period outgrowth was observed across the posterior capsule. The rate of cell coverage was dependent upon both species and the presence or absence of serum. The proliferative activity of the cells was greatest at or near the leading edge and decreased once covered. Wrinkles became visible in the posterior capsule during the late stages of precoverage and increased in both number and complexity. All cells on both the human and bovine posterior capsules contained F-actin and vimentin and the majority were immunolabelled for α-smooth muscle actin (α-SMA). Conclusions - This model exhibits many of the in vivo characteristics of the lens capsule after extracapsular surgery and may prove useful in further elucidating the cellular mechanisms of posterior capsule opacification.

Original languageEnglish (US)
Pages (from-to)945-952
Number of pages8
JournalBritish Journal of Ophthalmology
Volume82
Issue number8
StatePublished - Aug 1998
Externally publishedYes

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Capsulorhexis
Metaplasia
Lenses
Capsules
Cell Movement
Epithelial Cells
Cell Proliferation
Actins
Posterior Capsule of the Lens
Capsule Opacification
Organ Culture Techniques
Vimentin
Silicones
Smooth Muscle
Serum

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lens epithelial cell proliferation, migration, and metaplasia following capsulorhexis. / Saxby, Lisa; Rosen, Emanuel; Boulton, Mike.

In: British Journal of Ophthalmology, Vol. 82, No. 8, 08.1998, p. 945-952.

Research output: Contribution to journalArticle

Saxby, Lisa ; Rosen, Emanuel ; Boulton, Mike. / Lens epithelial cell proliferation, migration, and metaplasia following capsulorhexis. In: British Journal of Ophthalmology. 1998 ; Vol. 82, No. 8. pp. 945-952.
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