Leptin production in adipocytes from morbidly obese subjects: Stimulation by dexamethasone, inhibition with troglitazone, and influence of gender

Lloyd B. Williams, Rachael L. Fawcett, Angela S. Waechter, Peili Zhang, Brian E. Kogon, Rose Marie Jones, Margaret Inman, John Huse, Robert V. Considine

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

This study examined the regulation of leptin production by dexamethasone and troglitazone. Subcutaneous and omental adipose tissue was obtained during bariatric surgical procedures (30 women and 16 men; body mass index, 52.5 ± 1.7 kg/m2, age, 39± 2 yr), and adipocytes were cultured in suspension. Subcutaneous adipocytes from females released significantly more leptin than did omental cells from the same subject (P < 0.05), but basal leptin release was not different in adipocytes from these depots in males. Dexamethasone (0.1 μmol/L) significantly increased leptin release within 24 h from sc (135 ± 13% of control) and omental (227 ± 53%) adipocytes of females, but not males. Dexamethasone-stimulated leptin production at 48 h was significantly greater in the omental adipocytes of females (398 ± 64% of control) than in sc adipocytes of females (207 ± 21%) or the omental (211 ± 33%) and sc (180 ± 23%) adipocytes of males. Troglitazone (10 μmol/L; 48 h) significantly inhibited dexamethasone-stimulated leptin release in sc (57 ± 10.7% inhibition) and omental adipocytes (134 ± 26% inhibition). There was no gender-related difference in the effect of troglitazone to inhibit dexamethasone-stimulated leptin release. Troglitazone significantly inhibited basal leptin production from omental adipocytes by 15.0 ± 5.2%. The effect of dexamethasone and troglitazone to regulate leptin release was mediated through changes in ob gene expression, but did not involve changes in glucose uptake or metabolism to lactate. The data suggest that adipocytes from females are more responsive to the stimulatory effect of dexamethasone in vitro than are adipocytes from males. If adipocytes from females are more responsive to relevant in vivo stimuli for leptin secretion such as insulin or glucose, this could contribute to the gender difference in serum leptin. The data also suggest that leptin release from omental adipocytes may be more responsive to hormonal and nutrient regulation in vivo than are sc adipocytes.

Original languageEnglish (US)
Pages (from-to)2678-2684
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume85
Issue number8
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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