Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women

Shasha Meng, Shaowei Wu, Liming Liang, Geyu Liang, Edward Giovannucci, Immaculata De Vivo, Hongmei Nan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives: To examine the association between leukocyte mitochondrial DNA copy number (mtCN) and different anthropometric indices as well as weight changes; and to compare mtCN and telomere length with respect to their associations with BMI and age. Design: Population based cohort study. Setting: Nurses' Health Study, an ongoing prospective cohort study of 121,700 nurses enrolled in 1976; in 1989-1990 a subset of 32,826 women provided blood samples. Participants: 1,700 disease-free US women from case-control studies nested within the Nurses' Health Study with mtCN and telomere length measured who also have anthropometric measurements. Main outcome measure: Relative mtCN and telomere lengths in peripheral blood leukocytes measured by quantitative real time polymerase chain reaction and various anthropometric measurements data from initial questionnaire. Results: Leukocyte mtCN was inversely associated with current weight (LS means Q1-Q4: 0.07, 0.04, 0.03, -0.17; Ptrend =0.002), waist size (LS means Q1-Q4: 0.06, 0.05, -0.04, -0.06; Ptrend = 0.04), BMI (LS means normal light, normal heavy, overweight, pre-obese, obese: 0.11, -0.01, -0.04, 0.04, -0.25; Ptrend < 0.0001), and waist-hip ratio (WHR) (LS means Q1-Q4: 0.06, 0.08, -0.04, -0.06; Ptrend = 0.03). A one-unit decrease in mtCN z score was equivalent to approximately 3.5 pounds of weight gain for an adult of 5'10''. In addition, weight gain was bi-directionally and inversely associated with mtCN. Moreover, mtCN was strongly positively correlated with telomere length (LS means Q1-Q4: -0.02, 0.09, 0.11, 0.33; Ptrend < 0.0001). MtCN was inversely associated with BMI even after adjusting for telomere length (Ptrend =0.003), while telomere length was not associated with BMI. On the other hand, telomere length was inversely associated with age after adjusting for mtCN (Ptrend =0.04), while mtCN was not associated with age. Conclusions: Our results provide compelling evidence for a potential bi-directional temporal relationship between mitochondrial-mediated oxidative stress-defense mechanisms and weight change.

Original languageEnglish (US)
Pages (from-to)60676-60686
Number of pages11
JournalOncotarget
Volume7
Issue number37
DOIs
StatePublished - Jan 1 2016

Fingerprint

Mitochondrial DNA
Leukocytes
Telomere
Weights and Measures
Nurses
Weight Gain
Cohort Studies
Waist-Hip Ratio
Health
Case-Control Studies
Real-Time Polymerase Chain Reaction
Oxidative Stress
Outcome Assessment (Health Care)
Prospective Studies
Light

Keywords

  • Anthropometric indices
  • Mitochondrial DNA copy number
  • Telemere length
  • Weight change

ASJC Scopus subject areas

  • Oncology

Cite this

Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women. / Meng, Shasha; Wu, Shaowei; Liang, Liming; Liang, Geyu; Giovannucci, Edward; De Vivo, Immaculata; Nan, Hongmei.

In: Oncotarget, Vol. 7, No. 37, 01.01.2016, p. 60676-60686.

Research output: Contribution to journalArticle

Meng, S, Wu, S, Liang, L, Liang, G, Giovannucci, E, De Vivo, I & Nan, H 2016, 'Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women', Oncotarget, vol. 7, no. 37, pp. 60676-60686. https://doi.org/10.18632/oncotarget.10325
Meng S, Wu S, Liang L, Liang G, Giovannucci E, De Vivo I et al. Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women. Oncotarget. 2016 Jan 1;7(37):60676-60686. https://doi.org/10.18632/oncotarget.10325
Meng, Shasha ; Wu, Shaowei ; Liang, Liming ; Liang, Geyu ; Giovannucci, Edward ; De Vivo, Immaculata ; Nan, Hongmei. / Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women. In: Oncotarget. 2016 ; Vol. 7, No. 37. pp. 60676-60686.
@article{979305d860d04d869bc291c86fac5c79,
title = "Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women",
abstract = "Objectives: To examine the association between leukocyte mitochondrial DNA copy number (mtCN) and different anthropometric indices as well as weight changes; and to compare mtCN and telomere length with respect to their associations with BMI and age. Design: Population based cohort study. Setting: Nurses' Health Study, an ongoing prospective cohort study of 121,700 nurses enrolled in 1976; in 1989-1990 a subset of 32,826 women provided blood samples. Participants: 1,700 disease-free US women from case-control studies nested within the Nurses' Health Study with mtCN and telomere length measured who also have anthropometric measurements. Main outcome measure: Relative mtCN and telomere lengths in peripheral blood leukocytes measured by quantitative real time polymerase chain reaction and various anthropometric measurements data from initial questionnaire. Results: Leukocyte mtCN was inversely associated with current weight (LS means Q1-Q4: 0.07, 0.04, 0.03, -0.17; Ptrend =0.002), waist size (LS means Q1-Q4: 0.06, 0.05, -0.04, -0.06; Ptrend = 0.04), BMI (LS means normal light, normal heavy, overweight, pre-obese, obese: 0.11, -0.01, -0.04, 0.04, -0.25; Ptrend < 0.0001), and waist-hip ratio (WHR) (LS means Q1-Q4: 0.06, 0.08, -0.04, -0.06; Ptrend = 0.03). A one-unit decrease in mtCN z score was equivalent to approximately 3.5 pounds of weight gain for an adult of 5'10''. In addition, weight gain was bi-directionally and inversely associated with mtCN. Moreover, mtCN was strongly positively correlated with telomere length (LS means Q1-Q4: -0.02, 0.09, 0.11, 0.33; Ptrend < 0.0001). MtCN was inversely associated with BMI even after adjusting for telomere length (Ptrend =0.003), while telomere length was not associated with BMI. On the other hand, telomere length was inversely associated with age after adjusting for mtCN (Ptrend =0.04), while mtCN was not associated with age. Conclusions: Our results provide compelling evidence for a potential bi-directional temporal relationship between mitochondrial-mediated oxidative stress-defense mechanisms and weight change.",
keywords = "Anthropometric indices, Mitochondrial DNA copy number, Telemere length, Weight change",
author = "Shasha Meng and Shaowei Wu and Liming Liang and Geyu Liang and Edward Giovannucci and {De Vivo}, Immaculata and Hongmei Nan",
year = "2016",
month = "1",
day = "1",
doi = "10.18632/oncotarget.10325",
language = "English (US)",
volume = "7",
pages = "60676--60686",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "37",

}

TY - JOUR

T1 - Leukocyte mitochondrial DNA copy number, anthropometric indices, and weight change in US women

AU - Meng, Shasha

AU - Wu, Shaowei

AU - Liang, Liming

AU - Liang, Geyu

AU - Giovannucci, Edward

AU - De Vivo, Immaculata

AU - Nan, Hongmei

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Objectives: To examine the association between leukocyte mitochondrial DNA copy number (mtCN) and different anthropometric indices as well as weight changes; and to compare mtCN and telomere length with respect to their associations with BMI and age. Design: Population based cohort study. Setting: Nurses' Health Study, an ongoing prospective cohort study of 121,700 nurses enrolled in 1976; in 1989-1990 a subset of 32,826 women provided blood samples. Participants: 1,700 disease-free US women from case-control studies nested within the Nurses' Health Study with mtCN and telomere length measured who also have anthropometric measurements. Main outcome measure: Relative mtCN and telomere lengths in peripheral blood leukocytes measured by quantitative real time polymerase chain reaction and various anthropometric measurements data from initial questionnaire. Results: Leukocyte mtCN was inversely associated with current weight (LS means Q1-Q4: 0.07, 0.04, 0.03, -0.17; Ptrend =0.002), waist size (LS means Q1-Q4: 0.06, 0.05, -0.04, -0.06; Ptrend = 0.04), BMI (LS means normal light, normal heavy, overweight, pre-obese, obese: 0.11, -0.01, -0.04, 0.04, -0.25; Ptrend < 0.0001), and waist-hip ratio (WHR) (LS means Q1-Q4: 0.06, 0.08, -0.04, -0.06; Ptrend = 0.03). A one-unit decrease in mtCN z score was equivalent to approximately 3.5 pounds of weight gain for an adult of 5'10''. In addition, weight gain was bi-directionally and inversely associated with mtCN. Moreover, mtCN was strongly positively correlated with telomere length (LS means Q1-Q4: -0.02, 0.09, 0.11, 0.33; Ptrend < 0.0001). MtCN was inversely associated with BMI even after adjusting for telomere length (Ptrend =0.003), while telomere length was not associated with BMI. On the other hand, telomere length was inversely associated with age after adjusting for mtCN (Ptrend =0.04), while mtCN was not associated with age. Conclusions: Our results provide compelling evidence for a potential bi-directional temporal relationship between mitochondrial-mediated oxidative stress-defense mechanisms and weight change.

AB - Objectives: To examine the association between leukocyte mitochondrial DNA copy number (mtCN) and different anthropometric indices as well as weight changes; and to compare mtCN and telomere length with respect to their associations with BMI and age. Design: Population based cohort study. Setting: Nurses' Health Study, an ongoing prospective cohort study of 121,700 nurses enrolled in 1976; in 1989-1990 a subset of 32,826 women provided blood samples. Participants: 1,700 disease-free US women from case-control studies nested within the Nurses' Health Study with mtCN and telomere length measured who also have anthropometric measurements. Main outcome measure: Relative mtCN and telomere lengths in peripheral blood leukocytes measured by quantitative real time polymerase chain reaction and various anthropometric measurements data from initial questionnaire. Results: Leukocyte mtCN was inversely associated with current weight (LS means Q1-Q4: 0.07, 0.04, 0.03, -0.17; Ptrend =0.002), waist size (LS means Q1-Q4: 0.06, 0.05, -0.04, -0.06; Ptrend = 0.04), BMI (LS means normal light, normal heavy, overweight, pre-obese, obese: 0.11, -0.01, -0.04, 0.04, -0.25; Ptrend < 0.0001), and waist-hip ratio (WHR) (LS means Q1-Q4: 0.06, 0.08, -0.04, -0.06; Ptrend = 0.03). A one-unit decrease in mtCN z score was equivalent to approximately 3.5 pounds of weight gain for an adult of 5'10''. In addition, weight gain was bi-directionally and inversely associated with mtCN. Moreover, mtCN was strongly positively correlated with telomere length (LS means Q1-Q4: -0.02, 0.09, 0.11, 0.33; Ptrend < 0.0001). MtCN was inversely associated with BMI even after adjusting for telomere length (Ptrend =0.003), while telomere length was not associated with BMI. On the other hand, telomere length was inversely associated with age after adjusting for mtCN (Ptrend =0.04), while mtCN was not associated with age. Conclusions: Our results provide compelling evidence for a potential bi-directional temporal relationship between mitochondrial-mediated oxidative stress-defense mechanisms and weight change.

KW - Anthropometric indices

KW - Mitochondrial DNA copy number

KW - Telemere length

KW - Weight change

UR - http://www.scopus.com/inward/record.url?scp=84991299002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991299002&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.10325

DO - 10.18632/oncotarget.10325

M3 - Article

C2 - 27367031

AN - SCOPUS:84991299002

VL - 7

SP - 60676

EP - 60686

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 37

ER -