Levels of bimatoprost acid in the aqueous humour after bimatoprost treatment of patients with cataract

Louis B. Cantor, Joni Hoop, Darrell Wudunn, Chi Wah Yung, Yara Catoira, Shailaja Valluri, Arnold Cortes, Andrew Acheampong, David F. Woodward, Larry A. Wheeler

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25 Scopus citations

Abstract

Aim: To determine the aqueous humour concentration of the acid hydrolysis products of bimatoprost and latanoprost after a single topical dose of bimatoprost 0.03% or latanoprost 0.005% in humans. Methods: Randomised, controlled, double-masked, prospective study. 48 eyes of 48 patients scheduled for routine cataract surgery were randomised in an 8:2:2 ratio to treatment with a single 30 |il drop of bimatoprost 0.03%, latanoprost 0.005% or placebo at 1, 3, 6 or 1 2 h before the scheduled cataract surgery. Aqueous humour samples were withdrawn at the beginning of the surgical procedure and analysed using high-performance liquid chromatography-tandem mass spectrometry. Results: Bimatoprost acid (17-phenyl trinor prostaglandin F2α) was detected in aqueous samples at a mean concentration of 5.0 nM at hour 1, 6.7 nM at hour 3 and 1.9 nM at hour 6 after bimatoprost treatment. After latanoprost treatment, the mean concentration of latanoprost acid (13,14-dihydro-17-phenyl trinor prostaglandin F2α) in aqueous samples was 29.1 nM at hour 1, 41.3 nM at hour 3 and 2.5 nM at hour 6. Acid metabolites were below the limit of quantitation in all samples taken 12 h after dosing and in all samples from placebo-treated patients. None of the samples from latanoprost-treated patients contained quantifiable levels of non-metabolised latanoprost. Non-metabolised bimatoprost was detected in aqueous samples at a mean concentration of 6.6 nM at hour 1 and 2.4 nM at hour 3 after bimatoprost treatment. Conclusions: Low levels of bimatoprost acid were detected in aqueous humour samples from patients with cataract treated with a single dose of bimatoprost. Latanoprost acid concentrations in samples from patients treated with latanoprost were at least sixfold higher. These results suggest that bimatoprost acid in the aqueous humour does not sufficiently account for the ocular hypotensive efficacy of bimatoprost.

Original languageEnglish (US)
Pages (from-to)629-632
Number of pages4
JournalBritish Journal of Ophthalmology
Volume91
Issue number5
DOIs
StatePublished - May 2007

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Cantor, L. B., Hoop, J., Wudunn, D., Yung, C. W., Catoira, Y., Valluri, S., Cortes, A., Acheampong, A., Woodward, D. F., & Wheeler, L. A. (2007). Levels of bimatoprost acid in the aqueous humour after bimatoprost treatment of patients with cataract. British Journal of Ophthalmology, 91(5), 629-632. https://doi.org/10.1136/bjo.2006.110155