Levels of glutamate, aspartate, GABA, and taurine in different regions of the cerebellum after X-irradiation-induced neuronal loss

M. A. Rea, W. J. McBride, B. H. Rohde

Research output: Contribution to journalArticle

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Abstract

The levels of glutamate (Glu), aspartate (Asp), γ-amino-n-butyric acid (GABA), and taurine (Tau) were determined in the cortex, molecular layer, and deep nuclei of cerebella of adult rats exposed to X-irradiation at 12-15 days following birth (to prevent the acquisition of late-forming granule cells; 12-15x group) and 8-15 days following birth (to prevent the acquisition of granule and stellate cells; 8-15x group). Also, the levels of the four amino acids were measured in the crude synaptosomal fraction (P2) isolated from the whole cerebella of the control, 12-15x, and 8-15x groups. The level of Glu was significantly decreased by (1) 6-20% in the cerebellar cortex; (2) 15-20% in the molecular layer; and (3) 25-50% in the P2 fraction of the X-irradiated groups relative to control values. The content of Glu in the deep nuclei was not changed by X-irradiation treatment. Regional levels of Asp were unchanged by X-irradiation, while its level in P2 decreased by 15-30% after treatment. The levels of GABA and Tau in the molecular layer, deep nuclei, or P2 were not changed in the experimental groups. However, there was a 15% increase in the levels of GABA and Tau in the cerebellar cortex of the 8-15x group relative to control values. The data support the proposed role of glutamate as the excitatory transmitter released from the cerebellar granule cells but are inconclusive regarding a transmitter role for either Tau or GABA from cerebellar stellate cells.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalNeurochemical Research
Volume6
Issue number1
DOIs
StatePublished - Jan 1981

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Taurine
Aspartic Acid
Cerebellum
gamma-Aminobutyric Acid
Glutamic Acid
Irradiation
Cerebellar Cortex
Transmitters
Parturition
Butyric Acid
Rats
Amino Acids

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

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Levels of glutamate, aspartate, GABA, and taurine in different regions of the cerebellum after X-irradiation-induced neuronal loss. / Rea, M. A.; McBride, W. J.; Rohde, B. H.

In: Neurochemical Research, Vol. 6, No. 1, 01.1981, p. 33-39.

Research output: Contribution to journalArticle

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