Levels of growth factors from platelet-rich fibrin from chronic periodontitis versus periodontally healthy subjects: a pilot study

Jennifer Chang, Steven B. Blanchard, L. Jack Windsor, Richard L. Gregory, Yusuke Hamada

Research output: Contribution to journalArticle

Abstract

Objectives: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). Materials and methods: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. Results: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). Conclusions: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. Clinical relevance: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.

Original languageEnglish (US)
JournalClinical Oral Investigations
DOIs
StatePublished - Jan 1 2019

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Chronic Periodontitis
Fibrin
Intercellular Signaling Peptides and Proteins
Healthy Volunteers
Blood Platelets
Exudates and Transudates
Blood Cell Count
Interleukin-1
Membranes
Interleukin-6
Leukocytes
Tumor Necrosis Factor-alpha
Serum
Transforming Growth Factors
Somatomedins
Platelet Count
Leukocyte Count
Epidermal Growth Factor
Trypsin
Vascular Endothelial Growth Factor A

Keywords

  • Chronic periodontitis
  • Growth factors
  • Platelet concentrates
  • Platelet-rich fibrin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

@article{be323d170ead4586adb8a20312dc6783,
title = "Levels of growth factors from platelet-rich fibrin from chronic periodontitis versus periodontally healthy subjects: a pilot study",
abstract = "Objectives: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). Materials and methods: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. Results: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). Conclusions: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. Clinical relevance: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.",
keywords = "Chronic periodontitis, Growth factors, Platelet concentrates, Platelet-rich fibrin",
author = "Jennifer Chang and Blanchard, {Steven B.} and Windsor, {L. Jack} and Gregory, {Richard L.} and Yusuke Hamada",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s00784-019-02944-7",
language = "English (US)",
journal = "Clinical Oral Investigations",
issn = "1432-6981",
publisher = "Springer Verlag",

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TY - JOUR

T1 - Levels of growth factors from platelet-rich fibrin from chronic periodontitis versus periodontally healthy subjects

T2 - a pilot study

AU - Chang, Jennifer

AU - Blanchard, Steven B.

AU - Windsor, L. Jack

AU - Gregory, Richard L.

AU - Hamada, Yusuke

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objectives: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). Materials and methods: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. Results: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). Conclusions: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. Clinical relevance: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.

AB - Objectives: This study aimed to (1) compare the amounts of growth factors from platelet-rich fibrin (PRF) between chronic periodontitis and periodontally healthy subjects and (2) evaluate the relationships between the amounts of growth factors from PRF with complete blood counts (white blood cell (WBC) and platelet counts) and the serum concentrations of IL-1β, IL-6, and tumor necrosis factor-α (TNF-α). Materials and methods: Venous blood was collected from chronic periodontitis (test) and periodontally healthy subjects (control). PRF and serum were collected from the centrifuged blood. Liquid exudates from the compression of PRF were collected. The compressed PRF membranes were incubated in saline, and eluted aliquots were collected at 1, 24, and 72 h, and the membranes were then digested with trypsin. Epidermal growth factor, insulin-like growth factor-1, platelet-derived growth factor-BB, transforming growth factor-β1, and vascular endothelial growth factor in the exudates and eluents were quantified by ELISA. Serum was used for IL-1β, IL-6, and TNF-α quantifications. Complete blood counts were measured. Results: There were no significant differences in the amounts of growth factors from PRF exudates and membranes measured between groups (all p > 0.05). The test group had significantly higher WBC (p < 0.05). However, there was no significant correlation between the WBC and the amounts of the growth factors from PRF (all p > 0.05). Conclusions: PRF can be utilized as an autologous source of growth factors not affected by periodontal condition and WBC level. Clinical relevance: The amounts of growth factors from PRF were not affected by the periodontal condition of the patient.

KW - Chronic periodontitis

KW - Growth factors

KW - Platelet concentrates

KW - Platelet-rich fibrin

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U2 - 10.1007/s00784-019-02944-7

DO - 10.1007/s00784-019-02944-7

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JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

SN - 1432-6981

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