Light chain cardiomyopathy: Structural analysis of the light chain tissue deposits

Gloria Gallo, Fernando Goñi, Fuoad Boctor, Ruben Vidal, Asok Kumar, Fred J. Stevens, Blas Frangione, Jorge Ghiso

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Cardiomyopathy due to monoclonal light chain deposits is a complication of plasma cell disorders. The deposits may be either fibrillar as in light chain amyloid or nonfibrillar as in light chain deposition disease. The reasons for these structural differences are still unknown. We characterized the myocardial deposits by immunohistochemical examination of sections and extraction and biochemical analysis of the tissue deposits in a patient (MCM) who died of myeloma and systemic light chain deposition disease. Amino acid sequence analysis of the extracted nonfibrillar MCM κ-light chain reveals that it belongs to the L12a germline subset of the κ1 protein and contains five distinctive amino acid substitutions (three in the framework region III and two in the complementarity-determining region III) that have not been reported previously in the same positions in other κ1 light chains. The theoretically determined isoelectric point (pI 821) of the MCM light chain is high compared with the low isoelectric point of other Bence Jones proteins from subjects without light chain deposition disease. The diffuse binding to basement membranes and the high isoelectric point of the MCM κ-light chain suggest electrostatic interaction as a possible mechanism of tissue deposition. The spatial locations of the five distinctive residues and a sixth rare substitution of the MCM protein modeled on the backbone structure of REI, a K1-soluble Bence Jones light chain of known three-dimensional structure, may be responsible for protein destabilization, partial unfolding, and aggregation leading to tissue deposition.

Original languageEnglish (US)
Pages (from-to)1397-1406
Number of pages10
JournalAmerican Journal of Pathology
Volume148
Issue number5
StatePublished - May 1996
Externally publishedYes

Fingerprint

Cardiomyopathies
Light
Isoelectric Point
Bence Jones Protein
Complementarity Determining Regions
Proteins
Protein Sequence Analysis
Amino Acid Substitution
Plasma Cells
Static Electricity
Amyloid
Basement Membrane

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Gallo, G., Goñi, F., Boctor, F., Vidal, R., Kumar, A., Stevens, F. J., ... Ghiso, J. (1996). Light chain cardiomyopathy: Structural analysis of the light chain tissue deposits. American Journal of Pathology, 148(5), 1397-1406.

Light chain cardiomyopathy : Structural analysis of the light chain tissue deposits. / Gallo, Gloria; Goñi, Fernando; Boctor, Fuoad; Vidal, Ruben; Kumar, Asok; Stevens, Fred J.; Frangione, Blas; Ghiso, Jorge.

In: American Journal of Pathology, Vol. 148, No. 5, 05.1996, p. 1397-1406.

Research output: Contribution to journalArticle

Gallo, G, Goñi, F, Boctor, F, Vidal, R, Kumar, A, Stevens, FJ, Frangione, B & Ghiso, J 1996, 'Light chain cardiomyopathy: Structural analysis of the light chain tissue deposits', American Journal of Pathology, vol. 148, no. 5, pp. 1397-1406.
Gallo G, Goñi F, Boctor F, Vidal R, Kumar A, Stevens FJ et al. Light chain cardiomyopathy: Structural analysis of the light chain tissue deposits. American Journal of Pathology. 1996 May;148(5):1397-1406.
Gallo, Gloria ; Goñi, Fernando ; Boctor, Fuoad ; Vidal, Ruben ; Kumar, Asok ; Stevens, Fred J. ; Frangione, Blas ; Ghiso, Jorge. / Light chain cardiomyopathy : Structural analysis of the light chain tissue deposits. In: American Journal of Pathology. 1996 ; Vol. 148, No. 5. pp. 1397-1406.
@article{18f51ae1b2964c63a3c8abb312ce9b22,
title = "Light chain cardiomyopathy: Structural analysis of the light chain tissue deposits",
abstract = "Cardiomyopathy due to monoclonal light chain deposits is a complication of plasma cell disorders. The deposits may be either fibrillar as in light chain amyloid or nonfibrillar as in light chain deposition disease. The reasons for these structural differences are still unknown. We characterized the myocardial deposits by immunohistochemical examination of sections and extraction and biochemical analysis of the tissue deposits in a patient (MCM) who died of myeloma and systemic light chain deposition disease. Amino acid sequence analysis of the extracted nonfibrillar MCM κ-light chain reveals that it belongs to the L12a germline subset of the κ1 protein and contains five distinctive amino acid substitutions (three in the framework region III and two in the complementarity-determining region III) that have not been reported previously in the same positions in other κ1 light chains. The theoretically determined isoelectric point (pI 821) of the MCM light chain is high compared with the low isoelectric point of other Bence Jones proteins from subjects without light chain deposition disease. The diffuse binding to basement membranes and the high isoelectric point of the MCM κ-light chain suggest electrostatic interaction as a possible mechanism of tissue deposition. The spatial locations of the five distinctive residues and a sixth rare substitution of the MCM protein modeled on the backbone structure of REI, a K1-soluble Bence Jones light chain of known three-dimensional structure, may be responsible for protein destabilization, partial unfolding, and aggregation leading to tissue deposition.",
author = "Gloria Gallo and Fernando Go{\~n}i and Fuoad Boctor and Ruben Vidal and Asok Kumar and Stevens, {Fred J.} and Blas Frangione and Jorge Ghiso",
year = "1996",
month = "5",
language = "English (US)",
volume = "148",
pages = "1397--1406",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Light chain cardiomyopathy

T2 - Structural analysis of the light chain tissue deposits

AU - Gallo, Gloria

AU - Goñi, Fernando

AU - Boctor, Fuoad

AU - Vidal, Ruben

AU - Kumar, Asok

AU - Stevens, Fred J.

AU - Frangione, Blas

AU - Ghiso, Jorge

PY - 1996/5

Y1 - 1996/5

N2 - Cardiomyopathy due to monoclonal light chain deposits is a complication of plasma cell disorders. The deposits may be either fibrillar as in light chain amyloid or nonfibrillar as in light chain deposition disease. The reasons for these structural differences are still unknown. We characterized the myocardial deposits by immunohistochemical examination of sections and extraction and biochemical analysis of the tissue deposits in a patient (MCM) who died of myeloma and systemic light chain deposition disease. Amino acid sequence analysis of the extracted nonfibrillar MCM κ-light chain reveals that it belongs to the L12a germline subset of the κ1 protein and contains five distinctive amino acid substitutions (three in the framework region III and two in the complementarity-determining region III) that have not been reported previously in the same positions in other κ1 light chains. The theoretically determined isoelectric point (pI 821) of the MCM light chain is high compared with the low isoelectric point of other Bence Jones proteins from subjects without light chain deposition disease. The diffuse binding to basement membranes and the high isoelectric point of the MCM κ-light chain suggest electrostatic interaction as a possible mechanism of tissue deposition. The spatial locations of the five distinctive residues and a sixth rare substitution of the MCM protein modeled on the backbone structure of REI, a K1-soluble Bence Jones light chain of known three-dimensional structure, may be responsible for protein destabilization, partial unfolding, and aggregation leading to tissue deposition.

AB - Cardiomyopathy due to monoclonal light chain deposits is a complication of plasma cell disorders. The deposits may be either fibrillar as in light chain amyloid or nonfibrillar as in light chain deposition disease. The reasons for these structural differences are still unknown. We characterized the myocardial deposits by immunohistochemical examination of sections and extraction and biochemical analysis of the tissue deposits in a patient (MCM) who died of myeloma and systemic light chain deposition disease. Amino acid sequence analysis of the extracted nonfibrillar MCM κ-light chain reveals that it belongs to the L12a germline subset of the κ1 protein and contains five distinctive amino acid substitutions (three in the framework region III and two in the complementarity-determining region III) that have not been reported previously in the same positions in other κ1 light chains. The theoretically determined isoelectric point (pI 821) of the MCM light chain is high compared with the low isoelectric point of other Bence Jones proteins from subjects without light chain deposition disease. The diffuse binding to basement membranes and the high isoelectric point of the MCM κ-light chain suggest electrostatic interaction as a possible mechanism of tissue deposition. The spatial locations of the five distinctive residues and a sixth rare substitution of the MCM protein modeled on the backbone structure of REI, a K1-soluble Bence Jones light chain of known three-dimensional structure, may be responsible for protein destabilization, partial unfolding, and aggregation leading to tissue deposition.

UR - http://www.scopus.com/inward/record.url?scp=0029939217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029939217&partnerID=8YFLogxK

M3 - Article

C2 - 8623912

AN - SCOPUS:0029939217

VL - 148

SP - 1397

EP - 1406

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 5

ER -