Light chain composition of CSF oligoclonal IgG bands in multiple sclerosis and subacute sclerosing penecephalitis

David H. Mattson, Raymond P. Roos, John E. Hopper, Barry G.W. Arnason

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The light chain composition of MS and SSPE CSF oligoclonal IgG bands was examined using isoelectric focusing and sensitive peroxidase-anteperoxidase (PAP) staining technique specific for gamma heavy chains (γ), kappa light chains (κ), or lambda light chains (λ) and a radioimmunoassay (RIA) for γ, κ, or λ. Many bands in the 7 MS and 4 SSPE CSF examined were monoclonal, staining for either IgG-κ or IgG-λ. By staining, all MS CSF were κ predominant; SSPE CSF were variously κ orλ predominant. RIA confirmed the κ predominance of MS CSF. Three MS and 2 SSPE CSF contained bands staining for λ alone, i.e. free light chains. Analysis of RIA data confirmed these findings in 2 MS cases. The difference in light chain predominance of MS and SSPE CSF may reflect differences in the antigenic target, or the age of patient at the time when band-synthesizing clones are triggered. Six of 7 MS and all 4 SSPE CSF contained oligoclonal bands staining for γ and for both κ and λ, probably representing artifacts of IEF. No predominant immunochemical differences between bands in MS and SSPE were detected.

Original languageEnglish (US)
Pages (from-to)63-76
Number of pages14
JournalJournal of Neuroimmunology
Volume3
Issue number1
DOIs
StatePublished - Aug 1982
Externally publishedYes

Keywords

  • Cerebrospinal fluid
  • IgG bands
  • Light chain composition
  • Multiple sclerosis
  • Radioimmunoassay
  • Subacute sclerosing panencephalitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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