Limited nucleosome migration can completely randomize DNA repair patches in intact human cells

Gregory E. Arnold, A. Dunker, Michael J. Smerdon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Following irradiation of human cells with ultraviolet light, DNA repair patches are initially inserted near the 5′ and 3′ ends of nucleosome core DNA leaving a "gap" in repair synthesis (of ~ 50 bases) near the center of the core DNA. With time, however, these same repair patches become randomized, apparently by nucleosome migration. We have developed both an analytical expression and a computer algorithm (which simulates nucleosome migration along DNA) to determine the average distance nucleosomes must migrate to change the initial, non-uniform distribution of repair patches in nucleosomes to a random distribution. Both of these methods yielded the same result: nucleosomes must migrate an average of about 50 base-pairs in either direction to produce the randomization observed.

Original languageEnglish (US)
Pages (from-to)433-436
Number of pages4
JournalJournal of Molecular Biology
Volume196
Issue number2
DOIs
StatePublished - Jul 20 1987
Externally publishedYes

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Nucleosomes
DNA Repair
DNA
Ultraviolet Rays
Random Allocation
Base Pairing

ASJC Scopus subject areas

  • Virology

Cite this

Limited nucleosome migration can completely randomize DNA repair patches in intact human cells. / Arnold, Gregory E.; Dunker, A.; Smerdon, Michael J.

In: Journal of Molecular Biology, Vol. 196, No. 2, 20.07.1987, p. 433-436.

Research output: Contribution to journalArticle

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