Lipid and metabolite profiles of human brain tumors by desorption electrospray ionization-MS

Alan K. Jarmusch, Valentina Pirro, Zane Baird, Eyas M. Hattab, Aaron Cohen-Gadol, R. Graham Cooks

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Examination of tissue sections using desorption electrospray ionization (DESI)-MS revealed phospholipid-derived signals that differ between gray matter, white matter, gliomas, meningiomas, and pituitary tumors, allowing their ready discrimination by multivariate statistics. A set of lower mass signals, some corresponding to oncometabolites, including 2-hydroxyglutaric acid and N-acetylaspartic acid, was also observed in the DESI mass spectra, and these data further assisted in discrimination between brain parenchyma and gliomas. The combined information from the lipid and metabolite MS profiles recorded by DESI-MS and explored using multivariate statistics allowed successful differentiation of gray matter (n = 223), white matter (n = 66), gliomas (n = 158), meningiomas (n = 111), and pituitary tumors (n = 154) from 58 patients. A linear discriminant model used to distinguish brain parenchyma and gliomas yielded an overall sensitivity of 97.4% and a specificity of 98.5%. Furthermore, a discriminant model was created for tumor types (i.e., glioma, meningioma, and pituitary), which were discriminated with an overall sensitivity of 99.4% and a specificity of 99.7%. Unsupervised multivariate statistics were used to explore the chemical differences between anatomical regions of brain parenchyma and secondary infiltration. Infiltration of gliomas into normal tissue can be detected by DESI-MS. One hurdle to implementation of DESI-MS intraoperatively is the need for tissue freezing and sectioning, which we address by analyzing smeared biopsy tissue. Tissue smears are shown to give the same chemical information as tissue sections, eliminating the need for sectioning before MS analysis. These results lay the foundation for implementation of intraoperative DESI-MS evaluation of tissue smears for rapid diagnosis.

Original languageEnglish (US)
Pages (from-to)1486-1491
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number6
DOIs
StatePublished - Feb 9 2016

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Brain Neoplasms
Glioma
Lipids
Meningioma
Pituitary Neoplasms
Brain
Freezing
Linear Models
Phospholipids
Biopsy
Neoplasms

Keywords

  • Ambient ionization
  • MS imaging
  • Multivariate statistics
  • Neurosurgery
  • Pathology

ASJC Scopus subject areas

  • General

Cite this

Lipid and metabolite profiles of human brain tumors by desorption electrospray ionization-MS. / Jarmusch, Alan K.; Pirro, Valentina; Baird, Zane; Hattab, Eyas M.; Cohen-Gadol, Aaron; Cooks, R. Graham.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 6, 09.02.2016, p. 1486-1491.

Research output: Contribution to journalArticle

Jarmusch, Alan K. ; Pirro, Valentina ; Baird, Zane ; Hattab, Eyas M. ; Cohen-Gadol, Aaron ; Cooks, R. Graham. / Lipid and metabolite profiles of human brain tumors by desorption electrospray ionization-MS. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 6. pp. 1486-1491.
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