Lipooligosaccharide epitopes shared among Gram-negative non-enteric mucosal pathogens

Anthony A. Campagnari, Stanley Spinola, Alan J. Lesse, Yousef Abu Kwaik, Robert E. Mandrell, Michael A. Apicella

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The non-enteric Gram-negative human pathogens, B. catarrhalis, H. ducreyi, H. influenzae, N. gonorrhoeae and N. meningitidis, do not have repeating O-antigens as part of their principle surface glycolipid, the lipooligosaccharide (LOS). Because they have similar LOS structures, we studied the conservation of LOS oligosaccharide epitopes among these organisms. Twentyone monoclonal antibodies (mAbs) generated by immunizing mice with H. influenzae, N. gonorrhoeae and N. meningitidis were studied for cross reactivity. Five mAbs generated against non-typable H. influenzae were the only strain-specific antibodies. Ten mAbs reacted to LOS epitope(s) common to a genera or species, and six mAbs bound to epitope(s) on the LOS of strains from different genera. Some cross reactive mAbs bound to LOS bands of similar molecular weights, while others bound to bands of varying molecular weights. mAb 3F11, whose epitope mimics a human blood-group antigen, bound to a 4.8 kDa LOS band in N. gonorrhoeae and H. ducreyi, two pathogens that infect genital epithelium. mAb 3D9, whose epitope consists of 2-keto-3-deoxyoctulosonic acid (KDO), reacted with different LOS bands in N. gonorrhoeae, H. influenzae and some R mutants of S. minnesota. A 14 kb restriction fragment containing lipooligosaccharide synthesis genes responsible for the assembly of the 3D9 epitope in H. influenzae hybridized to all H. influenzae strains tested but did not hybridize to gonococcal and S. minnesota strains that expressed this epitope. These studies demonstrate that conserved LOS epitope(s) exist among different species and genera of non-enteric human pathogens and that different genetic mechanisms may have evolved in these pathogens to assemble some of these conserved epitopes.

Original languageEnglish (US)
Pages (from-to)353-362
Number of pages10
JournalMicrobial Pathogenesis
Volume8
Issue number5
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Epitopes
Human Influenza
Gonorrhea
Monoclonal Antibodies
Molecular Weight
lipid-linked oligosaccharides
O Antigens
Glycolipids
Blood Group Antigens
Oligosaccharides
Epithelium
Antibodies
Genes

Keywords

  • Branhamella
  • epitopes
  • Haemophilus
  • lipooligosaccharides
  • Neisseria

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Lipooligosaccharide epitopes shared among Gram-negative non-enteric mucosal pathogens. / Campagnari, Anthony A.; Spinola, Stanley; Lesse, Alan J.; Kwaik, Yousef Abu; Mandrell, Robert E.; Apicella, Michael A.

In: Microbial Pathogenesis, Vol. 8, No. 5, 1990, p. 353-362.

Research output: Contribution to journalArticle

Campagnari, Anthony A. ; Spinola, Stanley ; Lesse, Alan J. ; Kwaik, Yousef Abu ; Mandrell, Robert E. ; Apicella, Michael A. / Lipooligosaccharide epitopes shared among Gram-negative non-enteric mucosal pathogens. In: Microbial Pathogenesis. 1990 ; Vol. 8, No. 5. pp. 353-362.
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