Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated

R. E. Mandrell, R. McLaughlin, Y. A. Kwaik, A. Lesse, R. Yamasaki, B. Gibson, Stanley Spinola, M. A. Apicella

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The lipooligosaccharides (LOS) of strains of Haemophilus ducreyi, Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica contain epitopes that are antigenically and structurally similar to carbohydrates present in human glycosphingolipids. LOS from strains of Haemophilus influenzae and H. influenzae biogroup aegyptius were tested for the binding of monoclonal antibodies (MAbs) that bind to human glycosphingolipids possessing Galβ1-4GlcNAc (MAb 3F11) and Galα1-4Galβ1- 4Glc (MAb anti-P(k)). In solid-phase radioimmunoassays, the LOS of 18 of 19 H. influenzae type b (Hib), 8 of 19 nontypeable H. influenzae, and 10 of 20 H. influenzae biogroup aegyptius strains bound MAb anti-P(k). The LOS of 13 of 19 Hib, 10 of 16 nontypeable H. influenzae, and 2 of 18 H. influenzae biogroup aegyptius strains bound MAb 3F11. Neuraminidase treatment of the strains increased the binding of MAb 3F11 by more than twofold in 47% of the H. influenzae strains, suggesting that sialic acid occluded the LOS structure recognized by MAb 3F11. The material released from neuraminidase-treated Hib LOS was confirmed to be sialic acid by high-performance anion-exchange chromatography. A recombinant plasmid containing genes involved in Hib LOS biosynthesis directed the expression (assembly) of the 3F11 epitope in Escherichia coli. These studies demonstrate that H. influenzae and H. influenzae biogroup aegyptius express at least two LOS epitopes that are similar to those present in human glycosphingolipids. Sialic acid was present on the LOS of some H. influenzae strains and prevented the binding of MAb 3F11 to its epitope. The oligosaccharide portion of sialylated LOS may also resemble sialylated oligosaccharides present in human glycosphingolipids (gangliosides).

Original languageEnglish (US)
Pages (from-to)1322-1328
Number of pages7
JournalInfection and Immunity
Volume60
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Haemophilus
Glycosphingolipids
Haemophilus influenzae
Haemophilus influenzae type b
Epitopes
N-Acetylneuraminic Acid
Neuraminidase
Oligosaccharides
Neisseria lactamica
Haemophilus ducreyi
lipid-linked oligosaccharides
Neisseria meningitidis
Neisseria gonorrhoeae
Gangliosides
Radioimmunoassay
Anions
Chromatography
Plasmids
Monoclonal Antibodies
Carbohydrates

ASJC Scopus subject areas

  • Immunology

Cite this

Mandrell, R. E., McLaughlin, R., Kwaik, Y. A., Lesse, A., Yamasaki, R., Gibson, B., ... Apicella, M. A. (1992). Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated. Infection and Immunity, 60(4), 1322-1328.

Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated. / Mandrell, R. E.; McLaughlin, R.; Kwaik, Y. A.; Lesse, A.; Yamasaki, R.; Gibson, B.; Spinola, Stanley; Apicella, M. A.

In: Infection and Immunity, Vol. 60, No. 4, 1992, p. 1322-1328.

Research output: Contribution to journalArticle

Mandrell, RE, McLaughlin, R, Kwaik, YA, Lesse, A, Yamasaki, R, Gibson, B, Spinola, S & Apicella, MA 1992, 'Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated', Infection and Immunity, vol. 60, no. 4, pp. 1322-1328.
Mandrell RE, McLaughlin R, Kwaik YA, Lesse A, Yamasaki R, Gibson B et al. Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated. Infection and Immunity. 1992;60(4):1322-1328.
Mandrell, R. E. ; McLaughlin, R. ; Kwaik, Y. A. ; Lesse, A. ; Yamasaki, R. ; Gibson, B. ; Spinola, Stanley ; Apicella, M. A. / Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated. In: Infection and Immunity. 1992 ; Vol. 60, No. 4. pp. 1322-1328.
@article{a71685c94c844f6f81af7b32497afeeb,
title = "Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated",
abstract = "The lipooligosaccharides (LOS) of strains of Haemophilus ducreyi, Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica contain epitopes that are antigenically and structurally similar to carbohydrates present in human glycosphingolipids. LOS from strains of Haemophilus influenzae and H. influenzae biogroup aegyptius were tested for the binding of monoclonal antibodies (MAbs) that bind to human glycosphingolipids possessing Galβ1-4GlcNAc (MAb 3F11) and Galα1-4Galβ1- 4Glc (MAb anti-P(k)). In solid-phase radioimmunoassays, the LOS of 18 of 19 H. influenzae type b (Hib), 8 of 19 nontypeable H. influenzae, and 10 of 20 H. influenzae biogroup aegyptius strains bound MAb anti-P(k). The LOS of 13 of 19 Hib, 10 of 16 nontypeable H. influenzae, and 2 of 18 H. influenzae biogroup aegyptius strains bound MAb 3F11. Neuraminidase treatment of the strains increased the binding of MAb 3F11 by more than twofold in 47{\%} of the H. influenzae strains, suggesting that sialic acid occluded the LOS structure recognized by MAb 3F11. The material released from neuraminidase-treated Hib LOS was confirmed to be sialic acid by high-performance anion-exchange chromatography. A recombinant plasmid containing genes involved in Hib LOS biosynthesis directed the expression (assembly) of the 3F11 epitope in Escherichia coli. These studies demonstrate that H. influenzae and H. influenzae biogroup aegyptius express at least two LOS epitopes that are similar to those present in human glycosphingolipids. Sialic acid was present on the LOS of some H. influenzae strains and prevented the binding of MAb 3F11 to its epitope. The oligosaccharide portion of sialylated LOS may also resemble sialylated oligosaccharides present in human glycosphingolipids (gangliosides).",
author = "Mandrell, {R. E.} and R. McLaughlin and Kwaik, {Y. A.} and A. Lesse and R. Yamasaki and B. Gibson and Stanley Spinola and Apicella, {M. A.}",
year = "1992",
language = "English (US)",
volume = "60",
pages = "1322--1328",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Lipooligosaccharides (LOS) of some Haemophilus species mimic human glycosphingolipids, and some LOS are sialylated

AU - Mandrell, R. E.

AU - McLaughlin, R.

AU - Kwaik, Y. A.

AU - Lesse, A.

AU - Yamasaki, R.

AU - Gibson, B.

AU - Spinola, Stanley

AU - Apicella, M. A.

PY - 1992

Y1 - 1992

N2 - The lipooligosaccharides (LOS) of strains of Haemophilus ducreyi, Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica contain epitopes that are antigenically and structurally similar to carbohydrates present in human glycosphingolipids. LOS from strains of Haemophilus influenzae and H. influenzae biogroup aegyptius were tested for the binding of monoclonal antibodies (MAbs) that bind to human glycosphingolipids possessing Galβ1-4GlcNAc (MAb 3F11) and Galα1-4Galβ1- 4Glc (MAb anti-P(k)). In solid-phase radioimmunoassays, the LOS of 18 of 19 H. influenzae type b (Hib), 8 of 19 nontypeable H. influenzae, and 10 of 20 H. influenzae biogroup aegyptius strains bound MAb anti-P(k). The LOS of 13 of 19 Hib, 10 of 16 nontypeable H. influenzae, and 2 of 18 H. influenzae biogroup aegyptius strains bound MAb 3F11. Neuraminidase treatment of the strains increased the binding of MAb 3F11 by more than twofold in 47% of the H. influenzae strains, suggesting that sialic acid occluded the LOS structure recognized by MAb 3F11. The material released from neuraminidase-treated Hib LOS was confirmed to be sialic acid by high-performance anion-exchange chromatography. A recombinant plasmid containing genes involved in Hib LOS biosynthesis directed the expression (assembly) of the 3F11 epitope in Escherichia coli. These studies demonstrate that H. influenzae and H. influenzae biogroup aegyptius express at least two LOS epitopes that are similar to those present in human glycosphingolipids. Sialic acid was present on the LOS of some H. influenzae strains and prevented the binding of MAb 3F11 to its epitope. The oligosaccharide portion of sialylated LOS may also resemble sialylated oligosaccharides present in human glycosphingolipids (gangliosides).

AB - The lipooligosaccharides (LOS) of strains of Haemophilus ducreyi, Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica contain epitopes that are antigenically and structurally similar to carbohydrates present in human glycosphingolipids. LOS from strains of Haemophilus influenzae and H. influenzae biogroup aegyptius were tested for the binding of monoclonal antibodies (MAbs) that bind to human glycosphingolipids possessing Galβ1-4GlcNAc (MAb 3F11) and Galα1-4Galβ1- 4Glc (MAb anti-P(k)). In solid-phase radioimmunoassays, the LOS of 18 of 19 H. influenzae type b (Hib), 8 of 19 nontypeable H. influenzae, and 10 of 20 H. influenzae biogroup aegyptius strains bound MAb anti-P(k). The LOS of 13 of 19 Hib, 10 of 16 nontypeable H. influenzae, and 2 of 18 H. influenzae biogroup aegyptius strains bound MAb 3F11. Neuraminidase treatment of the strains increased the binding of MAb 3F11 by more than twofold in 47% of the H. influenzae strains, suggesting that sialic acid occluded the LOS structure recognized by MAb 3F11. The material released from neuraminidase-treated Hib LOS was confirmed to be sialic acid by high-performance anion-exchange chromatography. A recombinant plasmid containing genes involved in Hib LOS biosynthesis directed the expression (assembly) of the 3F11 epitope in Escherichia coli. These studies demonstrate that H. influenzae and H. influenzae biogroup aegyptius express at least two LOS epitopes that are similar to those present in human glycosphingolipids. Sialic acid was present on the LOS of some H. influenzae strains and prevented the binding of MAb 3F11 to its epitope. The oligosaccharide portion of sialylated LOS may also resemble sialylated oligosaccharides present in human glycosphingolipids (gangliosides).

UR - http://www.scopus.com/inward/record.url?scp=0026587936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026587936&partnerID=8YFLogxK

M3 - Article

C2 - 1372291

AN - SCOPUS:0026587936

VL - 60

SP - 1322

EP - 1328

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 4

ER -