Liquid chromatography for iothalamate in biological samples

Rajiv Agarwal, Nina Vasavada, Shawn D. Chase

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have previously reported an iothalamate assay for the assessment of the glomerular filtration rate (GFR) that required a long column equilibration time and 22 min run time per sample. We now report a simpler assay that requires a run time of only 5.5 min and is more precise and accurate than the earlier technique. The mobile phase consisted of methanol-acetonitrile-50 mM sodium monobasic phosphate (10:5:85, v/v) at pH 4.4, pumped at a rate of 1.5 ml/min on a C18 reversed-phase column. Samples of plasma and urine were deproteinized with 1 volume of 4% perchloric acid or 9 volumes of 2% perchloric acid, respectively. No internal standard was used. The diode array detection system collected absorbance at 240 nm and the peak height areas of iothalamate were determined. The iothalamate peak appeared at 3.5 min. Detector response was linear over the range tested (10-2000 μg/ml). Within-run precision was

Original languageEnglish (US)
Pages (from-to)345-352
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume785
Issue number2
DOIs
StatePublished - Mar 5 2003

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Iothalamic Acid
Liquid chromatography
Liquid Chromatography
Assays
Glomerular Filtration Rate
Methanol
Diodes
Urine
Detectors
Plasmas
Perchloric Acid

Keywords

  • Iothalamate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Liquid chromatography for iothalamate in biological samples. / Agarwal, Rajiv; Vasavada, Nina; Chase, Shawn D.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 785, No. 2, 05.03.2003, p. 345-352.

Research output: Contribution to journalArticle

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