Live-animal imaging of renal function by multiphoton Microscopy

Kenneth Dunn, Timothy Sutton, Ruben M. Sandoval

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Intravital microscopy, microscopy of living animals, is a powerful research technique that combines the resolution and sensitivity found in microscopic studies of cultured cells with the relevance and systemic influences of cells in the context of the intact animal. The power of intravital microscopy has recently been extended with the development of multiphoton fluorescence microscopy systems capable of collecting optical sections from deep within the kidney at subcellular resolution, supporting high-resolution characterizations of the structure and function of glomeruli, tubules, and vasculature in the living kidney. Fluorescent probes are administered to an anesthetized, surgically prepared animal, followed by image acquisition for up to 3 hr. Images are transferred via a high-speed network to specialized computer systems for digital image analysis. This general approach can be used with different combinations of fluorescent probes to evaluate processes such as glomerular permeability, proximal tubule endocytosis, microvascular flow, vascular permeability, mitochondrial function, and cellular apoptosis/necrosis.

Fingerprint

Microscopy
Microscopic examination
Animals
Kidney
Fluorescent Dyes
Imaging techniques
Multiphoton Fluorescence Microscopy
HIgh speed networks
Image acquisition
Fluorescence microscopy
Computer Systems
Capillary Permeability
Endocytosis
Image analysis
Cultured Cells
Permeability
Computer systems
Research Design
Necrosis
Cells

Keywords

  • Fluorescence microscopy
  • In vivo microscopy
  • Intravital microscopy
  • Multiphoton microscopy

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Biochemistry
  • Histology
  • Medicine(all)

Cite this

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abstract = "Intravital microscopy, microscopy of living animals, is a powerful research technique that combines the resolution and sensitivity found in microscopic studies of cultured cells with the relevance and systemic influences of cells in the context of the intact animal. The power of intravital microscopy has recently been extended with the development of multiphoton fluorescence microscopy systems capable of collecting optical sections from deep within the kidney at subcellular resolution, supporting high-resolution characterizations of the structure and function of glomeruli, tubules, and vasculature in the living kidney. Fluorescent probes are administered to an anesthetized, surgically prepared animal, followed by image acquisition for up to 3 hr. Images are transferred via a high-speed network to specialized computer systems for digital image analysis. This general approach can be used with different combinations of fluorescent probes to evaluate processes such as glomerular permeability, proximal tubule endocytosis, microvascular flow, vascular permeability, mitochondrial function, and cellular apoptosis/necrosis.",
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