Live tissue viability and chemosensitivity assays using digital holographic motility contrast imaging

Ran An, John Turek, Daniela Matei, David Nolte

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Holographic optical coherence imaging is an en face form of optical coherence tomography that uses lowcoherence digital holography as a coherence gate to select light from a chosen depth inside scattering tissue. By acquiring successive holograms at a high camera frame rate at a fixed depth, dynamic speckle provides information concerning dynamic light scattering from intracellular motility. Motility contrast imaging (MCI) uses living motion as a label-free and functional biomarker. MCI provides a new form of viability assay and also is applicable for proliferation and cytotoxicity assays. The results presented here demonstrate that low-coherence digital holography can extract viability information from biologically relevant three-dimensional (3D) tissue based on multicellular tumor spheroids by moving beyond the format of two-dimensional cell culture used for conventional high-content analysis. This paper also demonstrates the use of MCI for chemosensitivity assays on tumor exgrafts of excised ovarian cancer tumors responding to standard-of-care cisplatin chemotherapy. This ex vivo application extends the applicability of MCI beyond 3D tissue culture grown in vitro.

Original languageEnglish
JournalApplied Optics
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2013

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locomotion
viability
Assays
Tissue
Imaging techniques
Tumors
tumors
Holography
holography
Tissue culture
Chemotherapy
biomarkers
Optical tomography
spheroids
Biomarkers
Holograms
Dynamic light scattering
chemotherapy
Cytotoxicity
Speckle

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Live tissue viability and chemosensitivity assays using digital holographic motility contrast imaging. / An, Ran; Turek, John; Matei, Daniela; Nolte, David.

In: Applied Optics, Vol. 52, No. 1, 01.01.2013.

Research output: Contribution to journalArticle

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