Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion

Jason J. Winnick, Zhibo An, Guillaume Kraft, Christopher J. Ramnanan, Jose M. Irimia, Marta Smith, Margaret Lautz, Peter Roach, Alan D. Cherrington

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine the effect of liver glycogen loading on net hepatic glycogen synthesis during hyperinsulinemia or hepatic portal vein glucose infusion in vivo. Liver glycogen levels were supercompensated (SCGly) in two groups (using intraportal fructose infusion) but not in two others (Gly) during hyperglycemic-normoinsulinemia. Following a 2-h control period during which fructose infusion was stopped, there was a 2-h experimental period in which the response to hyperglycemia plus either 4X basal insulin (INS) or portal vein glucose infusion (PoG) was measured. Increased hepatic glycogen reduced the percent of glucose taken up by the liver that was deposited in glycogen (74 ± 3 vs. 53 ± 5% in Gly+INS and SCGly+INS, respectively, and 72 ± 3 vs. 50 ± 6% in Gly+PoG and SCGly+ PoG, respectively). The reduction in liver glycogen synthesis in SCGly+INS was accompanied by a decrease in both insulin signaling and an increase in AMPK activation, whereas only the latter was observed in SCGly+PoG. These data indicate that liver glycogen loading impairs glycogen synthesis regardless of the signal used to stimulate it.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalDiabetes
Volume62
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Liver Glycogen
Hyperinsulinism
Glycogen
Glucose
Insulin
Portal Vein
Fructose
Cerebral Veins
AMP-Activated Protein Kinases
Hepatic Veins
Hyperglycemia
Liver

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Winnick, J. J., An, Z., Kraft, G., Ramnanan, C. J., Irimia, J. M., Smith, M., ... Cherrington, A. D. (2013). Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion. Diabetes, 62(1), 96-101. https://doi.org/10.2337/db11-1773

Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion. / Winnick, Jason J.; An, Zhibo; Kraft, Guillaume; Ramnanan, Christopher J.; Irimia, Jose M.; Smith, Marta; Lautz, Margaret; Roach, Peter; Cherrington, Alan D.

In: Diabetes, Vol. 62, No. 1, 01.2013, p. 96-101.

Research output: Contribution to journalArticle

Winnick, JJ, An, Z, Kraft, G, Ramnanan, CJ, Irimia, JM, Smith, M, Lautz, M, Roach, P & Cherrington, AD 2013, 'Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion', Diabetes, vol. 62, no. 1, pp. 96-101. https://doi.org/10.2337/db11-1773
Winnick, Jason J. ; An, Zhibo ; Kraft, Guillaume ; Ramnanan, Christopher J. ; Irimia, Jose M. ; Smith, Marta ; Lautz, Margaret ; Roach, Peter ; Cherrington, Alan D. / Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion. In: Diabetes. 2013 ; Vol. 62, No. 1. pp. 96-101.
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