Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia

Jack Brelstaff, Aviva M. Tolkovsky, Bernardino Ghetti, Michel Goedert, Maria Grazia Spillantini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Tau protein forms insoluble filamentous inclusions that are closely associated with nerve cell death in many neurodegenerative diseases. How neurons die in these tauopathies is unclear. We report that living neurons with tau inclusions from P301S-tau mice expose abnormally high amounts of phosphatidylserine because of the production of reactive oxygen species (ROS). Consequently, co-cultured phagocytes (BV2 cells or primary microglia) identify and phagocytose the living neurons, thereby engulfing insoluble tau inclusions. To facilitate engulfment, neurons induce contacting microglia to secrete the opsonin milk-fat-globule EGF-factor-8 (MFGE8) and nitric oxide (NO), whereas neurons with tau inclusions are rescued when MFGE8 or NO production is prevented. MFGE8 expression is elevated in transgenic P301S-tau mouse brains with tau inclusions and in tau inclusion-rich brain regions of several human tauopathies, indicating shared mechanisms of disease. Preventing phagocytosis of living neurons will preserve them for treatments that inhibit tau aggregation and toxicity. Brelstaff et al. report that live neurons containing aggregated tau externalize phosphatidylserine, activate microglia, and are phagocytosed. Preventing key steps in this pathway rescues living neurons. A similar phagocytic signal is found in human tauopathies. The authors propose that inhibiting phagocytosis may spare neurons with tau aggregates.

Original languageEnglish (US)
Pages (from-to)1939-1948.e4
JournalCell Reports
Volume24
Issue number8
DOIs
StatePublished - Aug 21 2018

Fingerprint

Phosphatidylserines
Microglia
Phagocytosis
Neurons
Tauopathies
Epidermal Growth Factor
Brain
Nitric Oxide
Living Wills
Opsonin Proteins
Neurodegenerative diseases
tau Proteins
Cell death
Phagocytes
Neurodegenerative Diseases
Toxicity
Reactive Oxygen Species
Cell Death
Agglomeration

Keywords

  • cell death
  • MFGE8
  • microglia
  • neurodegeneration
  • neuroinflammation
  • nitric oxide
  • phagocytosis
  • phosphatidylserine
  • tau
  • tauopathies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia. / Brelstaff, Jack; Tolkovsky, Aviva M.; Ghetti, Bernardino; Goedert, Michel; Spillantini, Maria Grazia.

In: Cell Reports, Vol. 24, No. 8, 21.08.2018, p. 1939-1948.e4.

Research output: Contribution to journalArticle

Brelstaff, Jack ; Tolkovsky, Aviva M. ; Ghetti, Bernardino ; Goedert, Michel ; Spillantini, Maria Grazia. / Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia. In: Cell Reports. 2018 ; Vol. 24, No. 8. pp. 1939-1948.e4.
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