Local cerebral glucose utilization rates in alcohol-naïve high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats

Jennifer E. Learn, Daniel G. Smith, William J. McBride, Lawrence Lumeng, Ting Kai Li

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The present study compared baseline local cerebral glucose utilization (LCGU) values within reward-relevant brain regions in alcohol-naïve, adult male high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats from replicate lines 1 and 2. Methods: 2-[14C]Deoxyglucose ([14C]2-DG) was injected (125 μCi/kg) intravenously during the rats' dark cycle. Timed arterial blood samples were collected over 45 min and assayed for glucose as well as [14C]2-DG content. Rats were then decapitated; their brains quickly removed and frozen in isopentane at -50°C. Coronal sections from each brain were apposed to film and exposed for 2 days. Image densities were analyzed using quantitative autoradiography. Results: Data were collected from several key limbic (nucleus accumbens, ventral tegmental area, olfactory tubercle, amygdala, hippocampus, ventral pallidum, and septum), basal ganglia, cortical (medial prefrontal, frontal, parietal, temporal, occipital, entorhinal, pyriform, and cingulate), and subcortical (thalamus, habenula, and superior colliculus) structures. Because there were no significant differences between the replicates within each drinking line, data from the two replicates were combined to determine drinking line differences. When both replicate lines were combined, there were trends toward higher (approximately 15%) LCGU rates in HAD (n = 15) versus LAD (n = 16) rats within the parietal and occipital cortices, but neither of these line differences reached statistical significance (p < 0.01). Conclusions: The findings suggested that, within the HAD and LAD replicate rat lines, the selection for alcohol preference did not lead to differences in functional brain activity, as measured with the 2-DG method.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume25
Issue number4
StatePublished - 2001

Fingerprint

Alcohol Drinking
Rats
Alcohols
Glucose
Brain
Drinking
Habenula
Occipital Lobe
Parietal Lobe
Ventral Tegmental Area
Superior Colliculi
Deoxyglucose
Nucleus Accumbens
Amygdala
Basal Ganglia
Autoradiography
Thalamus
Reward
Hippocampus
Blood

Keywords

  • 2-Deoxyglucose
  • Functional Imaging
  • High-Alcohol-Drinking Rats
  • Local Cerebral Glucose Utilization

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Local cerebral glucose utilization rates in alcohol-naïve high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats. / Learn, Jennifer E.; Smith, Daniel G.; McBride, William J.; Lumeng, Lawrence; Li, Ting Kai.

In: Alcoholism: Clinical and Experimental Research, Vol. 25, No. 4, 2001, p. 517-523.

Research output: Contribution to journalArticle

Learn, Jennifer E. ; Smith, Daniel G. ; McBride, William J. ; Lumeng, Lawrence ; Li, Ting Kai. / Local cerebral glucose utilization rates in alcohol-naïve high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats. In: Alcoholism: Clinical and Experimental Research. 2001 ; Vol. 25, No. 4. pp. 517-523.
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T1 - Local cerebral glucose utilization rates in alcohol-naïve high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats

AU - Learn, Jennifer E.

AU - Smith, Daniel G.

AU - McBride, William J.

AU - Lumeng, Lawrence

AU - Li, Ting Kai

PY - 2001

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N2 - Background: The present study compared baseline local cerebral glucose utilization (LCGU) values within reward-relevant brain regions in alcohol-naïve, adult male high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats from replicate lines 1 and 2. Methods: 2-[14C]Deoxyglucose ([14C]2-DG) was injected (125 μCi/kg) intravenously during the rats' dark cycle. Timed arterial blood samples were collected over 45 min and assayed for glucose as well as [14C]2-DG content. Rats were then decapitated; their brains quickly removed and frozen in isopentane at -50°C. Coronal sections from each brain were apposed to film and exposed for 2 days. Image densities were analyzed using quantitative autoradiography. Results: Data were collected from several key limbic (nucleus accumbens, ventral tegmental area, olfactory tubercle, amygdala, hippocampus, ventral pallidum, and septum), basal ganglia, cortical (medial prefrontal, frontal, parietal, temporal, occipital, entorhinal, pyriform, and cingulate), and subcortical (thalamus, habenula, and superior colliculus) structures. Because there were no significant differences between the replicates within each drinking line, data from the two replicates were combined to determine drinking line differences. When both replicate lines were combined, there were trends toward higher (approximately 15%) LCGU rates in HAD (n = 15) versus LAD (n = 16) rats within the parietal and occipital cortices, but neither of these line differences reached statistical significance (p < 0.01). Conclusions: The findings suggested that, within the HAD and LAD replicate rat lines, the selection for alcohol preference did not lead to differences in functional brain activity, as measured with the 2-DG method.

AB - Background: The present study compared baseline local cerebral glucose utilization (LCGU) values within reward-relevant brain regions in alcohol-naïve, adult male high-alcohol-drinking (HAD) and low-alcohol-drinking (LAD) rats from replicate lines 1 and 2. Methods: 2-[14C]Deoxyglucose ([14C]2-DG) was injected (125 μCi/kg) intravenously during the rats' dark cycle. Timed arterial blood samples were collected over 45 min and assayed for glucose as well as [14C]2-DG content. Rats were then decapitated; their brains quickly removed and frozen in isopentane at -50°C. Coronal sections from each brain were apposed to film and exposed for 2 days. Image densities were analyzed using quantitative autoradiography. Results: Data were collected from several key limbic (nucleus accumbens, ventral tegmental area, olfactory tubercle, amygdala, hippocampus, ventral pallidum, and septum), basal ganglia, cortical (medial prefrontal, frontal, parietal, temporal, occipital, entorhinal, pyriform, and cingulate), and subcortical (thalamus, habenula, and superior colliculus) structures. Because there were no significant differences between the replicates within each drinking line, data from the two replicates were combined to determine drinking line differences. When both replicate lines were combined, there were trends toward higher (approximately 15%) LCGU rates in HAD (n = 15) versus LAD (n = 16) rats within the parietal and occipital cortices, but neither of these line differences reached statistical significance (p < 0.01). Conclusions: The findings suggested that, within the HAD and LAD replicate rat lines, the selection for alcohol preference did not lead to differences in functional brain activity, as measured with the 2-DG method.

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