Localization and characterization of the calsequestrin-binding domain of triadin 1. Evidence for a charged β-strand in mediating the protein-protein interaction

Yvonne M. Kobayashi, Badr A. Alseikhan, Larry R. Jones

Research output: Contribution to journalArticle

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Abstract

Triadin is an integral membrane protein of the junctional sarcoplasmic reticulum that binds to the high capacity Ca2+-binding protein calsequestrin and anchors it to the ryanodine receptor. The lumenal domain of triadin contains multiple repeats of alternating lysine and glutamic acid residues, which have been defined as KEKE motifs and have been proposed to promote protein associations. Here we identified the specific residues of triadin responsible for binding to calsequestrin by mutational analysis of triadin 1, the major cardiac isoform. A series of deletional fusion proteins of triadin 1 was generated, and by using metabolically labeled calsequestrin in filter-overlay assays, the calsequestrin binding domain of triadin 1 was localized to a single KEKE motif comprised of 25 amino acids. Alanine mutagenesis within this motif demonstrated that the critical amino acids of triadin binding to calsequestrin are the even-numbered residues Lys210, Lys212, Glu214, Lys216, Gly218, Gln220, Lys222, and Lys224. Replacement of the odd-numbered residues within this motif by alanine had no effect on calsequestrin binding to triadin. The results suggest a model in which residues 210-224 of triadin form a β-strand, with the even-numbered residues in the strand interacting with charged residues of calsequestrin, stabilizing a 'polar zipper' that links the two proteins together. This small, highly charged β-strand of triadin may tether calsequestrin to the junctional face membrane, allowing calsequestrin to sequester Ca2+ in the vicinity of the ryanodine receptor during Ca2+ uptake and Ca2+ release.

Original languageEnglish (US)
Pages (from-to)17639-17646
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number23
DOIs
StatePublished - Jun 9 2000

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Calsequestrin
Proteins
Ryanodine Receptor Calcium Release Channel
Alanine
triadin
Amino Acids
Mutagenesis
Fasteners
Sarcoplasmic Reticulum
Anchors
Lysine
Glutamic Acid
Assays
Carrier Proteins
Protein Isoforms
Membrane Proteins
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Localization and characterization of the calsequestrin-binding domain of triadin 1. Evidence for a charged β-strand in mediating the protein-protein interaction. / Kobayashi, Yvonne M.; Alseikhan, Badr A.; Jones, Larry R.

In: Journal of Biological Chemistry, Vol. 275, No. 23, 09.06.2000, p. 17639-17646.

Research output: Contribution to journalArticle

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