Localization of calponin binding sites in the structure of 90 kDa heat shock protein (Hsp90)

Natalia Bogatcheva, Yushu Ma, Dunja Urosev, Nikolai B. Gusev

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The structure of rabbit liver Hsp90 was reevaluated by limited trypsinolysis, N-terminal sequencing and determination of the site that is phosphorylated by casein kinase II. Limited proteolysis results in formation of four groups of large peptides with M(r) in the range of 26-41 kDa. Peptides with M(r) 39-41 kDa were represented by large N-terminal and central peptides starting at residue 283 of the α-isoform of Hsp90. All sites phosphorylated by casein kinase II were located in the large 39-41 kDa peptides. Peptides with M(r) 26-27 kDa were represented by short N-terminal and central peptides starting at Glu-400 of the α-isoform of Hsp90. The data of affinity chromatography and light scattering indicate that smooth muscle calponin interacts with Hsp90. The calponin binding sites are located in the large (37-41 kDa) N-terminal and in a short (26-27 kDa) central peptide starting at Glu-400 of the α-isoform of Hsp90. Phosphorylation by casein kinase II up to 2 mol of phosphate per mol of Hsp90 does not affect interaction of Hsp90 with calponin. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)369-374
Number of pages6
JournalFEBS Letters
Volume457
Issue number3
DOIs
StatePublished - Sep 3 1999
Externally publishedYes

Fingerprint

HSP90 Heat-Shock Proteins
Heat-Shock Proteins
Casein Kinase II
Binding Sites
Peptides
Protein Isoforms
Affinity Chromatography
Proteolysis
Smooth Muscle
Affinity chromatography
Phosphates
Phosphorylation
Rabbits
Light
calponin
Liver
Light scattering
Muscle
retinal S antigen peptide M

Keywords

  • Calponin
  • Casein kinase
  • Heat shock protein
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization of calponin binding sites in the structure of 90 kDa heat shock protein (Hsp90). / Bogatcheva, Natalia; Ma, Yushu; Urosev, Dunja; Gusev, Nikolai B.

In: FEBS Letters, Vol. 457, No. 3, 03.09.1999, p. 369-374.

Research output: Contribution to journalArticle

Bogatcheva, Natalia ; Ma, Yushu ; Urosev, Dunja ; Gusev, Nikolai B. / Localization of calponin binding sites in the structure of 90 kDa heat shock protein (Hsp90). In: FEBS Letters. 1999 ; Vol. 457, No. 3. pp. 369-374.
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