Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer

Eri S. Srivatsan, Rita Chakrabarti, Kayvan Zainabadi, Svetlana D. Pack, Payam Benyamini, Marc Mendonca, Kwan Yang Pok, Kevin Kang, Daria Motamedi, Mark P. Sawicki, Zhengping Zhuang, Rachel A. Jesudasan, Ulla Bengtsson, Chi Sun, Bruce A. Roe, Eric J. Stanbridge, Sharon P. Wilczynski, J. Leslie Redpath

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Previous molecular genetic studies on HeLa cell (a cervical cancer cell line) derived non-tumorigenic and tumorigenic hybrids have localized a tumor suppressor gene to the long arm of chromosome 11. Analysis of cervical cancer cell lines using chromosome 11 specific probes showed deletion and translocation of 11q13 sequences in five out of eight cell lines. Fluorescence in situ hybridization (FISH), using 11q13 specific probes, has shown interstitial deletion of 11q13 sequences in the HeLa cells. In order to determine whether 11q13 deletions occur in primary cervical tumors, we analysed 36 tumors using 20 different microsatellite and RFLP markers. Semi automated fluorescein based allelotyping was performed to identify loss of heterozygosity (LOH) in tumors. The results showed allelic loss in 17 (47%) tumors. Three different regions of loss, one near MEN1, the second near D11S913, and the third near INT2 locus were observed. The smallest region of deletion overlap at the D11S913 locus was localized to a 300 Kb distance between D11S4908 and D11S5023. Fluorescence in situ hybridization (FISH), using 11q13 specific cosmid and BAC (bacterial artificial chromosome) probes, confirmed allelic deletion in the tumors. PCR analysis further identified homozygous deletion of 11q13 sequences in a primary tumor, in HeLa cells and in two HeLa cell derived tumorigenic hybrid cell lines. The homozygous deletion in the cell lines was mapped to a 5.7 kb sequence of 11q13. We hypothesize therefore that a putative cervical cancer tumor suppressor gene exists within the 300 kb of chromosome 11q13.

Original languageEnglish (US)
Pages (from-to)5631-5642
Number of pages12
JournalOncogene
Volume21
Issue number36
DOIs
StatePublished - Aug 15 2002

Fingerprint

Uterine Cervical Neoplasms
Chromosomes
HeLa Cells
Cell Line
Tumor Suppressor Genes
Neoplasms
Chromosomes, Human, Pair 11
Sequence Deletion
Loss of Heterozygosity
Fluorescence In Situ Hybridization
Multiple Endocrine Neoplasia Type 1
Bacterial Artificial Chromosomes
Cosmids
Hybrid Cells
Fluorescein
Restriction Fragment Length Polymorphisms
Microsatellite Repeats
Molecular Biology
Polymerase Chain Reaction

Keywords

  • Cervical cancer
  • Chromosome 11q13
  • Fluorescence in situ hybridization
  • Homozygous deletion
  • Loss of heterozygosity
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Srivatsan, E. S., Chakrabarti, R., Zainabadi, K., Pack, S. D., Benyamini, P., Mendonca, M., ... Redpath, J. L. (2002). Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. Oncogene, 21(36), 5631-5642. https://doi.org/10.1038/sj.onc.1205698

Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. / Srivatsan, Eri S.; Chakrabarti, Rita; Zainabadi, Kayvan; Pack, Svetlana D.; Benyamini, Payam; Mendonca, Marc; Pok, Kwan Yang; Kang, Kevin; Motamedi, Daria; Sawicki, Mark P.; Zhuang, Zhengping; Jesudasan, Rachel A.; Bengtsson, Ulla; Sun, Chi; Roe, Bruce A.; Stanbridge, Eric J.; Wilczynski, Sharon P.; Redpath, J. Leslie.

In: Oncogene, Vol. 21, No. 36, 15.08.2002, p. 5631-5642.

Research output: Contribution to journalArticle

Srivatsan, ES, Chakrabarti, R, Zainabadi, K, Pack, SD, Benyamini, P, Mendonca, M, Pok, KY, Kang, K, Motamedi, D, Sawicki, MP, Zhuang, Z, Jesudasan, RA, Bengtsson, U, Sun, C, Roe, BA, Stanbridge, EJ, Wilczynski, SP & Redpath, JL 2002, 'Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer', Oncogene, vol. 21, no. 36, pp. 5631-5642. https://doi.org/10.1038/sj.onc.1205698
Srivatsan ES, Chakrabarti R, Zainabadi K, Pack SD, Benyamini P, Mendonca M et al. Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. Oncogene. 2002 Aug 15;21(36):5631-5642. https://doi.org/10.1038/sj.onc.1205698
Srivatsan, Eri S. ; Chakrabarti, Rita ; Zainabadi, Kayvan ; Pack, Svetlana D. ; Benyamini, Payam ; Mendonca, Marc ; Pok, Kwan Yang ; Kang, Kevin ; Motamedi, Daria ; Sawicki, Mark P. ; Zhuang, Zhengping ; Jesudasan, Rachel A. ; Bengtsson, Ulla ; Sun, Chi ; Roe, Bruce A. ; Stanbridge, Eric J. ; Wilczynski, Sharon P. ; Redpath, J. Leslie. / Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. In: Oncogene. 2002 ; Vol. 21, No. 36. pp. 5631-5642.
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abstract = "Previous molecular genetic studies on HeLa cell (a cervical cancer cell line) derived non-tumorigenic and tumorigenic hybrids have localized a tumor suppressor gene to the long arm of chromosome 11. Analysis of cervical cancer cell lines using chromosome 11 specific probes showed deletion and translocation of 11q13 sequences in five out of eight cell lines. Fluorescence in situ hybridization (FISH), using 11q13 specific probes, has shown interstitial deletion of 11q13 sequences in the HeLa cells. In order to determine whether 11q13 deletions occur in primary cervical tumors, we analysed 36 tumors using 20 different microsatellite and RFLP markers. Semi automated fluorescein based allelotyping was performed to identify loss of heterozygosity (LOH) in tumors. The results showed allelic loss in 17 (47{\%}) tumors. Three different regions of loss, one near MEN1, the second near D11S913, and the third near INT2 locus were observed. The smallest region of deletion overlap at the D11S913 locus was localized to a 300 Kb distance between D11S4908 and D11S5023. Fluorescence in situ hybridization (FISH), using 11q13 specific cosmid and BAC (bacterial artificial chromosome) probes, confirmed allelic deletion in the tumors. PCR analysis further identified homozygous deletion of 11q13 sequences in a primary tumor, in HeLa cells and in two HeLa cell derived tumorigenic hybrid cell lines. The homozygous deletion in the cell lines was mapped to a 5.7 kb sequence of 11q13. We hypothesize therefore that a putative cervical cancer tumor suppressor gene exists within the 300 kb of chromosome 11q13.",
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T1 - Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer

AU - Srivatsan, Eri S.

AU - Chakrabarti, Rita

AU - Zainabadi, Kayvan

AU - Pack, Svetlana D.

AU - Benyamini, Payam

AU - Mendonca, Marc

AU - Pok, Kwan Yang

AU - Kang, Kevin

AU - Motamedi, Daria

AU - Sawicki, Mark P.

AU - Zhuang, Zhengping

AU - Jesudasan, Rachel A.

AU - Bengtsson, Ulla

AU - Sun, Chi

AU - Roe, Bruce A.

AU - Stanbridge, Eric J.

AU - Wilczynski, Sharon P.

AU - Redpath, J. Leslie

PY - 2002/8/15

Y1 - 2002/8/15

N2 - Previous molecular genetic studies on HeLa cell (a cervical cancer cell line) derived non-tumorigenic and tumorigenic hybrids have localized a tumor suppressor gene to the long arm of chromosome 11. Analysis of cervical cancer cell lines using chromosome 11 specific probes showed deletion and translocation of 11q13 sequences in five out of eight cell lines. Fluorescence in situ hybridization (FISH), using 11q13 specific probes, has shown interstitial deletion of 11q13 sequences in the HeLa cells. In order to determine whether 11q13 deletions occur in primary cervical tumors, we analysed 36 tumors using 20 different microsatellite and RFLP markers. Semi automated fluorescein based allelotyping was performed to identify loss of heterozygosity (LOH) in tumors. The results showed allelic loss in 17 (47%) tumors. Three different regions of loss, one near MEN1, the second near D11S913, and the third near INT2 locus were observed. The smallest region of deletion overlap at the D11S913 locus was localized to a 300 Kb distance between D11S4908 and D11S5023. Fluorescence in situ hybridization (FISH), using 11q13 specific cosmid and BAC (bacterial artificial chromosome) probes, confirmed allelic deletion in the tumors. PCR analysis further identified homozygous deletion of 11q13 sequences in a primary tumor, in HeLa cells and in two HeLa cell derived tumorigenic hybrid cell lines. The homozygous deletion in the cell lines was mapped to a 5.7 kb sequence of 11q13. We hypothesize therefore that a putative cervical cancer tumor suppressor gene exists within the 300 kb of chromosome 11q13.

AB - Previous molecular genetic studies on HeLa cell (a cervical cancer cell line) derived non-tumorigenic and tumorigenic hybrids have localized a tumor suppressor gene to the long arm of chromosome 11. Analysis of cervical cancer cell lines using chromosome 11 specific probes showed deletion and translocation of 11q13 sequences in five out of eight cell lines. Fluorescence in situ hybridization (FISH), using 11q13 specific probes, has shown interstitial deletion of 11q13 sequences in the HeLa cells. In order to determine whether 11q13 deletions occur in primary cervical tumors, we analysed 36 tumors using 20 different microsatellite and RFLP markers. Semi automated fluorescein based allelotyping was performed to identify loss of heterozygosity (LOH) in tumors. The results showed allelic loss in 17 (47%) tumors. Three different regions of loss, one near MEN1, the second near D11S913, and the third near INT2 locus were observed. The smallest region of deletion overlap at the D11S913 locus was localized to a 300 Kb distance between D11S4908 and D11S5023. Fluorescence in situ hybridization (FISH), using 11q13 specific cosmid and BAC (bacterial artificial chromosome) probes, confirmed allelic deletion in the tumors. PCR analysis further identified homozygous deletion of 11q13 sequences in a primary tumor, in HeLa cells and in two HeLa cell derived tumorigenic hybrid cell lines. The homozygous deletion in the cell lines was mapped to a 5.7 kb sequence of 11q13. We hypothesize therefore that a putative cervical cancer tumor suppressor gene exists within the 300 kb of chromosome 11q13.

KW - Cervical cancer

KW - Chromosome 11q13

KW - Fluorescence in situ hybridization

KW - Homozygous deletion

KW - Loss of heterozygosity

KW - Tumor suppressor gene

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