Localization of Drosophila retinal degeneration B, a membrane-associated phosphatidylinositol transfer protein

Thomas S. Vihtelic, Mark Goebl, Scott Milligan, Joseph E. O'Tousa, David R. Hyde

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

The Drosophila retinal degeneration B (rdgB) mutation causes abnormal photoreceptor response and light-enhanced retinal degeneration. Immunoblots using polyclonal anti-rdgB serum showed that rdgB is a 160-kD membrane protein. The antiserum localized the rdgB protein in photoreceptors, antennae, and regions of the Drosophila brain, indicating that the rdgB protein functions in many sensory and neuronal cells. In photoreceptors, the protein localized adjacent to the rhabdomeres, in the vicinity of the subrhabdomeric cisternae. The rdgB protein's aminoterminal 281 residues are >40% identical to the rat brain phosphatidylinositol transfer protein (PI-TP). A truncated rdgB protein, which contains only this amino-terminal domain, possesses a phosphatidylinositol transfer activity in vitro. The remaining 773 carboxyl terminal amino acids have additional functional domains. Nitrocellulose overlay experiments reveal that an acidic amino acid domain, adjacent to the PI transfer domain, binds 45Ca+2. Six hydrophobic segments are found in the middle of the putative translation product and likely function as membrane spanning domains. These results suggest that the rdgB protein, unlike the small soluble PI-TPs, is a membrane-associated PI-TP, which may be directly regulated by light-induced changes in intracellular calcium.

Original languageEnglish
Pages (from-to)1013-1022
Number of pages10
JournalJournal of Cell Biology
Volume122
Issue number5
StatePublished - Sep 1993

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Phospholipid Transfer Proteins
Retinal Degeneration
Drosophila
Membrane Proteins
Acidic Amino Acids
Light
Collodion
Brain
Phosphatidylinositols
Immune Sera
Calcium
Amino Acids

ASJC Scopus subject areas

  • Cell Biology

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Localization of Drosophila retinal degeneration B, a membrane-associated phosphatidylinositol transfer protein. / Vihtelic, Thomas S.; Goebl, Mark; Milligan, Scott; O'Tousa, Joseph E.; Hyde, David R.

In: Journal of Cell Biology, Vol. 122, No. 5, 09.1993, p. 1013-1022.

Research output: Contribution to journalArticle

Vihtelic, Thomas S. ; Goebl, Mark ; Milligan, Scott ; O'Tousa, Joseph E. ; Hyde, David R. / Localization of Drosophila retinal degeneration B, a membrane-associated phosphatidylinositol transfer protein. In: Journal of Cell Biology. 1993 ; Vol. 122, No. 5. pp. 1013-1022.
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