Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse

Ju Chen, Changshun Shao, Virginie Lazar, Carolyn H. Srivastava, Wei Hua Lee, Jay A. Tischfield

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.

Original languageEnglish
Pages (from-to)369-375
Number of pages7
JournalJournal of Cellular Biochemistry
Volume64
Issue number3
DOIs
StatePublished - Mar 1 1997

Fingerprint

Phospholipases A2
Spermatocytes
Genes
Molecular Weight
Molecular weight
Transcription
Messenger RNA
Reverse Transcription
In Situ Hybridization
Complementary DNA
Seminiferous Epithelium
Polymerase Chain Reaction
Spermatids
Meiosis
Northern Blotting
Amplification
Testis
Nucleotides
RNA
Tissue

Keywords

  • cDNA
  • in situ hybridization
  • meiosis
  • mouse
  • phospholipase A
  • pla2g2c
  • spermatocytes
  • testis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse. / Chen, Ju; Shao, Changshun; Lazar, Virginie; Srivastava, Carolyn H.; Lee, Wei Hua; Tischfield, Jay A.

In: Journal of Cellular Biochemistry, Vol. 64, No. 3, 01.03.1997, p. 369-375.

Research output: Contribution to journalArticle

Chen, Ju ; Shao, Changshun ; Lazar, Virginie ; Srivastava, Carolyn H. ; Lee, Wei Hua ; Tischfield, Jay A. / Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse. In: Journal of Cellular Biochemistry. 1997 ; Vol. 64, No. 3. pp. 369-375.
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AU - Tischfield, Jay A.

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