Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse

Ju Chen, Changshun Shao, Virginie Lazar, Carolyn H. Srivastava, Wei Hua Lee, Jay A. Tischfield

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5' and 3' RACE (rapid amplification of cDNA ends) RT-PCR, and this nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII.

Original languageEnglish (US)
Pages (from-to)369-375
Number of pages7
JournalJournal of Cellular Biochemistry
Volume64
Issue number3
DOIs
StatePublished - Mar 1 1997

Keywords

  • cDNA
  • in situ hybridization
  • meiosis
  • mouse
  • phospholipase A
  • pla2g2c
  • spermatocytes
  • testis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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