Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms

B. Kluve-Beckerman, S. L. Naylor, A. Marshall, J. C. Gardner, T. B. Shows, Merrill Benson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A human serum amyloid A (SAA) cDNA was used as a probe in chromosome mapping studies to detect human SAA gene sequences in DNA isolated from human/mouse somatic cell hybrids. Southern analysis of DNA from 20 hybrid cell lines, including some with translocations of human chromosomes, placed the SAA gene(s) in the p11→pter region of chromosome 11. Screening of human DNA from unrelated individuals by Southern analysis using the SAA cDNA probe revealed restriction fragment polymorphisms for HindIII and PstI. An analysis of the segregation of these polymorphisms with other markers on the short arm of chromosome 11 should more precisely map the SAA gene(s).

Original languageEnglish
Pages (from-to)1196-1204
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number3
DOIs
StatePublished - Jun 30 1986

Fingerprint

Serum Amyloid A Protein
Chromosomes, Human, Pair 11
Chromosomes
Polymorphism
Genes
DNA
Hybrid Cells
Complementary DNA
Chromosome Mapping
Human Chromosomes
Screening
Cells
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms. / Kluve-Beckerman, B.; Naylor, S. L.; Marshall, A.; Gardner, J. C.; Shows, T. B.; Benson, Merrill.

In: Biochemical and Biophysical Research Communications, Vol. 137, No. 3, 30.06.1986, p. 1196-1204.

Research output: Contribution to journalArticle

Kluve-Beckerman, B. ; Naylor, S. L. ; Marshall, A. ; Gardner, J. C. ; Shows, T. B. ; Benson, Merrill. / Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms. In: Biochemical and Biophysical Research Communications. 1986 ; Vol. 137, No. 3. pp. 1196-1204.
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