Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms

B. Kluve-Beckerman, S. L. Naylor, A. Marshall, J. C. Gardner, T. B. Shows, M. D. Benson

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

A human serum amyloid A (SAA) cDNA was used as a probe in chromosome mapping studies to detect human SAA gene sequences in DNA isolated from human/mouse somatic cell hybrids. Southern analysis of DNA from 20 hybrid cell lines, including some with translocations of human chromosomes, placed the SAA gene(s) in the p11→pter region of chromosome 11. Screening of human DNA from unrelated individuals by Southern analysis using the SAA cDNA probe revealed restriction fragment polymorphisms for HindIII and PstI. An analysis of the segregation of these polymorphisms with other markers on the short arm of chromosome 11 should more precisely map the SAA gene(s).

Original languageEnglish (US)
Pages (from-to)1196-1204
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number3
DOIs
StatePublished - Jun 30 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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