Localization of phospholamban in slow but not fast canine skeletal muscle fibers. An immunocytochemical and biochemical study

A. O. Jorgensen, Larry Jones

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Phospholamban, originally described as a cardiac sarcoplasmic reticulum protein, was localized in cryostat sections of three adult canine skeletal muscles (gracilis, extensor carpi radialis, and superficial digitalis flexor) by immunofluorenscence labeling with highly specific phospholamban antibodies. Only some myofibers were strongly labeled with phospholamban antibodies. The labeling of myofibers with phospholamban antibodies was compared to the distribution of Type I (slow) and Type II (fast) myofibers as determined by staining adjacent sections cytochemically for the alkali-stable myosin ATPase, a specific marker for Type II myofibers. All the skeletal myofibers labeled for phospholamban above background levels corresponded to Type I (slow) myofibers. The presence of phospholamban in microsomal fractions isolated from canine superficial digitalis flexor (89 ± 3% Type I) and extensor carpi radialis skeletal muscle (14 ± 6% Type I) was confirmed by immunoblotting. Antiserum to cardiac phospholamban bound to proteins of apparent M(r) values of 25,000 (oligomeric phospholamban) and 5,000-6,000 (monomeric phospholamban) in sarcoplasmic reticulum vesicles from both muscles. Quantification of phospholamban in sarcoplasmic reticulum vesicles from cardic, slow, and fast skeletal muscle tisues following phosphorylation with [γ-32P]ATP suggested that superficial digitalis flexor and extensor carpi radialis skeletal muscle contained about 16 and 3%, respectively, as much phospholamban as cardiac muscle per unit of sarcoplasmic reticulum. The presence of phospholamban in both Type I (slow) and cardiac muscle fibers supports the possibility that the Ca2+ fluxes across the sarcoplasmic reticulum in both fiber types are similarly regulated, and is consistent with the idea that the relaxant effect of catecholamines on slow skeletal muscle is mediated in part by phosphorylation of phospholamban.

Original languageEnglish (US)
Pages (from-to)3775-3781
Number of pages7
JournalJournal of Biological Chemistry
Volume261
Issue number8
StatePublished - 1986
Externally publishedYes

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Skeletal Muscle Fibers
Muscle
Canidae
Fibers
Sarcoplasmic Reticulum
Skeletal Muscle
Digitalis
Phosphorylation
phospholamban
Labeling
Antibodies
Myocardium
Cryostats
Alkalies
Myosins
Immunoblotting
Catecholamines
Immune Sera
Proteins
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Localization of phospholamban in slow but not fast canine skeletal muscle fibers. An immunocytochemical and biochemical study. / Jorgensen, A. O.; Jones, Larry.

In: Journal of Biological Chemistry, Vol. 261, No. 8, 1986, p. 3775-3781.

Research output: Contribution to journalArticle

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abstract = "Phospholamban, originally described as a cardiac sarcoplasmic reticulum protein, was localized in cryostat sections of three adult canine skeletal muscles (gracilis, extensor carpi radialis, and superficial digitalis flexor) by immunofluorenscence labeling with highly specific phospholamban antibodies. Only some myofibers were strongly labeled with phospholamban antibodies. The labeling of myofibers with phospholamban antibodies was compared to the distribution of Type I (slow) and Type II (fast) myofibers as determined by staining adjacent sections cytochemically for the alkali-stable myosin ATPase, a specific marker for Type II myofibers. All the skeletal myofibers labeled for phospholamban above background levels corresponded to Type I (slow) myofibers. The presence of phospholamban in microsomal fractions isolated from canine superficial digitalis flexor (89 ± 3{\%} Type I) and extensor carpi radialis skeletal muscle (14 ± 6{\%} Type I) was confirmed by immunoblotting. Antiserum to cardiac phospholamban bound to proteins of apparent M(r) values of 25,000 (oligomeric phospholamban) and 5,000-6,000 (monomeric phospholamban) in sarcoplasmic reticulum vesicles from both muscles. Quantification of phospholamban in sarcoplasmic reticulum vesicles from cardic, slow, and fast skeletal muscle tisues following phosphorylation with [γ-32P]ATP suggested that superficial digitalis flexor and extensor carpi radialis skeletal muscle contained about 16 and 3{\%}, respectively, as much phospholamban as cardiac muscle per unit of sarcoplasmic reticulum. The presence of phospholamban in both Type I (slow) and cardiac muscle fibers supports the possibility that the Ca2+ fluxes across the sarcoplasmic reticulum in both fiber types are similarly regulated, and is consistent with the idea that the relaxant effect of catecholamines on slow skeletal muscle is mediated in part by phosphorylation of phospholamban.",
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