Localization of potential tumor suppressor loci to a < 2 Mb region on chromosome 17q in human prostate cancer

X. Gao, A. Zacharek, D. J. Grignon, W. Sakr, I. J. Powell, A. T. Porter, K. V. Honn

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

We recently demonstrated a high frequency of loss of heterozygosity (LOH) at the D17S856 and D17S855 (within the BRCA1 gene) loci in primary prostate cancer, suggesting that the BRCA1 gene and/or other tumor suppressor gene(s) located within the interval of the D17S856 and D17S855 loci and/or within the vicinity of this interval may be important in prostate cancer. To further define the exact boundary of the deleted region (i.e., D17S856/D17S855) and to detect other possible LOH regions on the long arm of chromosome 17, we analysed 23 matched normal and tumor DNAs with 15 polymorphic microsatellite markers spanning chromosome 17q12-21. Eleven of 22 (50%) informative tumors showed allelic deletion at one or more of the loci studied. A minimal area of LOH was identified to extend from the proximal boundary at the D17S776 locus to the distal boundary at the D17S855 locus, spanning an estimated < 2 Mb segment on chromosome 17q21. Our results suggest that a potential tumor suppressor gene(s) may reside in the < 2 Mb region centromeric (inclusive) to the BRCA1 gene and that this tumor suppressor gene(s) may be involved in the formation of prostate cancer.

Original languageEnglish (US)
Pages (from-to)1241-1247
Number of pages7
JournalOncogene
Volume11
Issue number7
StatePublished - Jan 1 1995

Keywords

  • Deletion mapping
  • Loss of heterozygosity
  • Polymerase chain reaction
  • Prostatic carcinoma
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Gao, X., Zacharek, A., Grignon, D. J., Sakr, W., Powell, I. J., Porter, A. T., & Honn, K. V. (1995). Localization of potential tumor suppressor loci to a < 2 Mb region on chromosome 17q in human prostate cancer. Oncogene, 11(7), 1241-1247.