Long-term AZT exposure alters the metabolic capacity of cultured human lymphoblastoid cells

Ofelia A. Olivero, Irma L. Vazquez, Catherine C. Cooch, Jessica Ming, Emily Keller, Mia Yu, Jennifer P. Borojerdi, Hannan M. Braun, Edward McKee, Miriam C. Poirier

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The antiretroviral efficacy of 3'-azido-3'-deoxythymidine (AZT) is dependent upon intracellular mono-, di-, and triphosphorylation and incorporation into DNA in place of thymidine. Thymidine kinase 1 (TK-1) catalyzes the first step of this pathway. MOLT-3, human lymphoblastoid cells, were exposed to AZT continuously for 14 passages (P1-P14) and cultured for an additional 14 passages (P15-P28) without AZT. Progressive and irreversible depletion of the enzymatically active form of the TK-1 24-kDa monomer with loss of active protein was demonstrated during P1-P5 of AZT exposure. From P15 to P28, both the 24- and the 48-kDa forms of TK-1 were undetectable and a tetrameric 96-kDa form was present. AZT-DNA incorporation was observed with values of 150, 133, and 108 molecules of AZT/106 nucleotides at the 10μM plasma-equivalent AZT dose at P1, P5, and P14, respectively. An exposure-related increase in the frequency of micronuclei (MN) was observed in cells exposed to either 10 or 800μM AZT during P1-P14. Analysis of the cell cycle profile revealed an accumulation of S-phase cells and a decrease in G1-phase cells during exposure to 800μM AZT for 14 passages. When MOLT-3 cells were grown in AZT-free media (P15-P29), there was a reduction in AZT-DNA incorporation and MN formation; however, TK-1 depletion and the persistence of S-phase delay were unchanged. These data suggest that in addition to known mutagenic mechanisms, cells may become resistant to AZT partially through inactivation of TK-1 and through modulation of cell cycle components.

Original languageEnglish (US)
Pages (from-to)109-117
Number of pages9
JournalToxicological Sciences
Volume115
Issue number1
DOIs
StatePublished - May 1 2010

Keywords

  • Antiretrovirals
  • Nucleoside analog
  • Thymidine kinase

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Long-term AZT exposure alters the metabolic capacity of cultured human lymphoblastoid cells'. Together they form a unique fingerprint.

  • Cite this

    Olivero, O. A., Vazquez, I. L., Cooch, C. C., Ming, J., Keller, E., Yu, M., Borojerdi, J. P., Braun, H. M., McKee, E., & Poirier, M. C. (2010). Long-term AZT exposure alters the metabolic capacity of cultured human lymphoblastoid cells. Toxicological Sciences, 115(1), 109-117. https://doi.org/10.1093/toxsci/kfq023