Long-term bone regeneration, mineralization and angiogenesis in rat calvarial defects implanted with strong porous bioactive glass (13-93) scaffolds

Yinan Lin, Wei Xiao, Xin Liu, B. Sonny Bal, Lynda Bonewald, Mohamed N. Rahaman

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

There is growing interest in the use of bioactive glass scaffolds for repairing structural bone defects but data on the capacity of the scaffolds to regenerate bone in vivo, particularly over a long-term duration, are limited. In this study, bone regeneration in rat calvarial defects implanted with strong porous scaffolds of silicate 13-93 glass (porosity = 47 ± 1%) was investigated at 12 and 24 weeks post-implantation and compared with previous results from a similar study at 6 weeks. Three groups of implants, composed of as-fabricated scaffolds, scaffolds pretreated in a phosphate solution to convert a thin surface layer (5 μm) to hydroxyapatite (HA) and pretreated scaffolds loaded with bone morphogenetic protein-2 (BMP2) (1 μg/defect) were used. Bone regeneration, bioactive glass conversion to HA and blood vessel formation in the defects implanted with the three groups of scaffolds were evaluated using histology, histomorphometric analysis and scanning electron microscopy. When compared to the as-fabricated scaffolds, the pretreated scaffolds enhanced bone regeneration at 6 weeks but not at 12 or 24 weeks. In comparison, the BMP2-loaded scaffolds showed a significantly better capacity to regenerate bone at all three implantation times and they were almost completely infiltrated with lamellar bone within 12 weeks. The amount of glass conversion to HA at 24 weeks (30-33%) was not significantly different among the three groups of scaffolds. The area and number of blood vessels in the new bone that infiltrated the BMP2-loaded scaffolds at 6 and 12 weeks postimplantation were significantly greater than those for the as-fabricated and pretreated scaffolds. However, there was no significant difference in blood vessel area and number among the three groups of scaffolds at 24 weeks. The results indicate that these strong porous bioactive glass (13-93) scaffolds loaded with BMP2 are promising candidate implants for structural bone repair.

Original languageEnglish (US)
Pages (from-to)120-129
Number of pages10
JournalJournal of Non-Crystalline Solids
Volume432
DOIs
StatePublished - Jan 15 2016
Externally publishedYes

Keywords

  • Angiogenesis
  • Bioactive glass scaffolds
  • Bone morphogenetic protein-2
  • Bone regeneration
  • Rat calvarial defect model

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

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