Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells

Arthur B. Raitano, Philip Scuderi, Murray Korc

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The long-term cytotoxic/cytostatic effects of recombinant human tumor necrosis factor alpha (rhTNF) and gamma interferon (rhIFN-7) were studied in five human pancreatic carcinoma cell lines. The pancreatic tumor cell lines were heterogenous in their response to individual cytokines. During a 7-d incubation, MIA PaCa-2 cells were more sensitive to rhTNF than rhIFN-γ, whereas ASPC-1, T3M4, and COLO 357 cells were more sensitive to rhIFN-γ than rhTNF. PANC-1 cells were relatively insensitive to both cytokines. In a previous report, we demonstrated synergistic cytotoxic effects of rhTNF and rhIFN-γ during 48-h incubations in ASPC-1 and COLO 357 cells (Raitano et al, Pancreas, April, 1990). In this study, a 7-d treatment with both rhTNF and rhIFN-γ did not produce synergistic effects in any of the cell lines. However, a 24-h treatment with rhIFN-γ, followed by removal of the cytokine, markedly increased the long-term cytotoxic/cytostatic effects of rhTNF in ASPC-1, COLO 357, and T3M4 cells. In contrast, a similar pretreatment with rhTNF did not increase the long-term cytotoxic/cytostatic effects of rhIFN-γ in any of the cell lines. These data suggest that, in some human pancreatic carcinoma cell lines, rhIFN-γ may be especially useful in the long-term suppression of growth. Furthermore, brief pulses of rhIFN-γ may also be especially efficacious when followed by a subsequent prolonged exposure of cells to rhTNF.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalInternational Journal of Pancreatology
Volume6
Issue number2
DOIs
StatePublished - Mar 1 1990

Keywords

  • Cytokines
  • HLA-DR
  • cytotoxicity
  • tumor cells

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells'. Together they form a unique fingerprint.

  • Cite this