Long-term intermittent high-amplitude subcutaneous nerve stimulation reduces sympathetic tone in ambulatory dogs

Yuan Yuan, Zhaolei Jiang, Ye Zhao, Wei Chung Tsai, Jheel Patel, Lan Chen, Changyu Shen, Shien-Fong Lin, Huei Sheng Vincent Chen, Thomas Everett, Michael C. Fishbein, Zhenhui Chen, Peng-Sheng Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Reducing sympathetic efferent outflow from the stellate ganglia (SG) may be antiarrhythmic. Objective: The purpose of this study was to test the hypothesis that chronic thoracic subcutaneous nerve stimulation (ScNS) could reduce SG nerve activity (SGNA) and control paroxysmal atrial tachycardia (PAT). Methods: Thoracic ScNS was performed in 8 dogs while SGNA, vagal nerve activity (VNA), and subcutaneous nerve activity (ScNA) were monitored. An additional 3 dogs were used for sham stimulation as controls. Results: Xinshu ScNS and left lateral thoracic nerve ScNS reduced heart rate (HR). Xinshu ScNS at 3.5 mA for 2 weeks reduced mean average SGNA from 5.32 μV (95% confidence interval [CI] 3.89–6.75) at baseline to 3.24 μV (95% CI 2.16–4.31; P =.015) and mean HR from 89 bpm (95% CI 80–98) at baseline to 83 bpm (95% CI 76–90; P =.007). Bilateral SG showed regions of decreased tyrosine hydroxylase staining with increased terminal deoxynucleotidyl transferase dUTP nick-end labeling–positive nuclei in 18.47% (95% CI 9.68–46.62) of all ganglion cells, indicating cell death. Spontaneous PAT episodes were reduced from 9.83 per day (95% CI 5.77–13.89) in controls to 3.00 per day (95% CI 0.11–5.89) after ScNS (P =.027). Left lateral thoracic nerve ScNS also led to significant bilateral SG neuronal death and significantly reduced average SGNA and HR in dogs. Conclusion: ScNS at 2 different sites in the thorax led to SG cell death, reduced SGNA, and suppressed PAT in ambulatory dogs.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalHeart Rhythm
Volume15
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

Stellate Ganglion
Thoracic Nerves
Dogs
Confidence Intervals
Paroxysmal Tachycardia
Heart Rate
Cell Death
DNA Nucleotidylexotransferase
Tyrosine 3-Monooxygenase
Ganglia
Thorax
Staining and Labeling

Keywords

  • Arrhythmia
  • Autonomic nervous system
  • Nerve Recording
  • Neuromodulation
  • Stellate ganglion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Long-term intermittent high-amplitude subcutaneous nerve stimulation reduces sympathetic tone in ambulatory dogs. / Yuan, Yuan; Jiang, Zhaolei; Zhao, Ye; Tsai, Wei Chung; Patel, Jheel; Chen, Lan; Shen, Changyu; Lin, Shien-Fong; Chen, Huei Sheng Vincent; Everett, Thomas; Fishbein, Michael C.; Chen, Zhenhui; Chen, Peng-Sheng.

In: Heart Rhythm, Vol. 15, No. 3, 01.03.2018, p. 451-459.

Research output: Contribution to journalArticle

Yuan, Yuan ; Jiang, Zhaolei ; Zhao, Ye ; Tsai, Wei Chung ; Patel, Jheel ; Chen, Lan ; Shen, Changyu ; Lin, Shien-Fong ; Chen, Huei Sheng Vincent ; Everett, Thomas ; Fishbein, Michael C. ; Chen, Zhenhui ; Chen, Peng-Sheng. / Long-term intermittent high-amplitude subcutaneous nerve stimulation reduces sympathetic tone in ambulatory dogs. In: Heart Rhythm. 2018 ; Vol. 15, No. 3. pp. 451-459.
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abstract = "Background: Reducing sympathetic efferent outflow from the stellate ganglia (SG) may be antiarrhythmic. Objective: The purpose of this study was to test the hypothesis that chronic thoracic subcutaneous nerve stimulation (ScNS) could reduce SG nerve activity (SGNA) and control paroxysmal atrial tachycardia (PAT). Methods: Thoracic ScNS was performed in 8 dogs while SGNA, vagal nerve activity (VNA), and subcutaneous nerve activity (ScNA) were monitored. An additional 3 dogs were used for sham stimulation as controls. Results: Xinshu ScNS and left lateral thoracic nerve ScNS reduced heart rate (HR). Xinshu ScNS at 3.5 mA for 2 weeks reduced mean average SGNA from 5.32 μV (95{\%} confidence interval [CI] 3.89–6.75) at baseline to 3.24 μV (95{\%} CI 2.16–4.31; P =.015) and mean HR from 89 bpm (95{\%} CI 80–98) at baseline to 83 bpm (95{\%} CI 76–90; P =.007). Bilateral SG showed regions of decreased tyrosine hydroxylase staining with increased terminal deoxynucleotidyl transferase dUTP nick-end labeling–positive nuclei in 18.47{\%} (95{\%} CI 9.68–46.62) of all ganglion cells, indicating cell death. Spontaneous PAT episodes were reduced from 9.83 per day (95{\%} CI 5.77–13.89) in controls to 3.00 per day (95{\%} CI 0.11–5.89) after ScNS (P =.027). Left lateral thoracic nerve ScNS also led to significant bilateral SG neuronal death and significantly reduced average SGNA and HR in dogs. Conclusion: ScNS at 2 different sites in the thorax led to SG cell death, reduced SGNA, and suppressed PAT in ambulatory dogs.",
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T1 - Long-term intermittent high-amplitude subcutaneous nerve stimulation reduces sympathetic tone in ambulatory dogs

AU - Yuan, Yuan

AU - Jiang, Zhaolei

AU - Zhao, Ye

AU - Tsai, Wei Chung

AU - Patel, Jheel

AU - Chen, Lan

AU - Shen, Changyu

AU - Lin, Shien-Fong

AU - Chen, Huei Sheng Vincent

AU - Everett, Thomas

AU - Fishbein, Michael C.

AU - Chen, Zhenhui

AU - Chen, Peng-Sheng

PY - 2018/3/1

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N2 - Background: Reducing sympathetic efferent outflow from the stellate ganglia (SG) may be antiarrhythmic. Objective: The purpose of this study was to test the hypothesis that chronic thoracic subcutaneous nerve stimulation (ScNS) could reduce SG nerve activity (SGNA) and control paroxysmal atrial tachycardia (PAT). Methods: Thoracic ScNS was performed in 8 dogs while SGNA, vagal nerve activity (VNA), and subcutaneous nerve activity (ScNA) were monitored. An additional 3 dogs were used for sham stimulation as controls. Results: Xinshu ScNS and left lateral thoracic nerve ScNS reduced heart rate (HR). Xinshu ScNS at 3.5 mA for 2 weeks reduced mean average SGNA from 5.32 μV (95% confidence interval [CI] 3.89–6.75) at baseline to 3.24 μV (95% CI 2.16–4.31; P =.015) and mean HR from 89 bpm (95% CI 80–98) at baseline to 83 bpm (95% CI 76–90; P =.007). Bilateral SG showed regions of decreased tyrosine hydroxylase staining with increased terminal deoxynucleotidyl transferase dUTP nick-end labeling–positive nuclei in 18.47% (95% CI 9.68–46.62) of all ganglion cells, indicating cell death. Spontaneous PAT episodes were reduced from 9.83 per day (95% CI 5.77–13.89) in controls to 3.00 per day (95% CI 0.11–5.89) after ScNS (P =.027). Left lateral thoracic nerve ScNS also led to significant bilateral SG neuronal death and significantly reduced average SGNA and HR in dogs. Conclusion: ScNS at 2 different sites in the thorax led to SG cell death, reduced SGNA, and suppressed PAT in ambulatory dogs.

AB - Background: Reducing sympathetic efferent outflow from the stellate ganglia (SG) may be antiarrhythmic. Objective: The purpose of this study was to test the hypothesis that chronic thoracic subcutaneous nerve stimulation (ScNS) could reduce SG nerve activity (SGNA) and control paroxysmal atrial tachycardia (PAT). Methods: Thoracic ScNS was performed in 8 dogs while SGNA, vagal nerve activity (VNA), and subcutaneous nerve activity (ScNA) were monitored. An additional 3 dogs were used for sham stimulation as controls. Results: Xinshu ScNS and left lateral thoracic nerve ScNS reduced heart rate (HR). Xinshu ScNS at 3.5 mA for 2 weeks reduced mean average SGNA from 5.32 μV (95% confidence interval [CI] 3.89–6.75) at baseline to 3.24 μV (95% CI 2.16–4.31; P =.015) and mean HR from 89 bpm (95% CI 80–98) at baseline to 83 bpm (95% CI 76–90; P =.007). Bilateral SG showed regions of decreased tyrosine hydroxylase staining with increased terminal deoxynucleotidyl transferase dUTP nick-end labeling–positive nuclei in 18.47% (95% CI 9.68–46.62) of all ganglion cells, indicating cell death. Spontaneous PAT episodes were reduced from 9.83 per day (95% CI 5.77–13.89) in controls to 3.00 per day (95% CI 0.11–5.89) after ScNS (P =.027). Left lateral thoracic nerve ScNS also led to significant bilateral SG neuronal death and significantly reduced average SGNA and HR in dogs. Conclusion: ScNS at 2 different sites in the thorax led to SG cell death, reduced SGNA, and suppressed PAT in ambulatory dogs.

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