Long-term subthreshold electrical stimulation of the left stellate ganglion and a canine model of sudden cardiac death

Moshe Swissa, Shengmei Zhou, Ignacio Gonzalez-Gomez, Che Ming Chang, Angela C. Lai, Adam W. Cates, Michael C. Fishbein, Hrayr S. Karagueuzian, Peng Sheng Chen, Lan S. Chen

Research output: Contribution to journalArticle

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Abstract

Objectives We sought to develop a high-yield canine model of sudden cardiac death (SCD). Background Because electrical stimulation is a powerful means to elicit nerve sprouting, we hypothesize that subthreshold electrical stimulation is more effective than nerve growth factor (NGF) infusion in inducing nerve sprouting and SCD in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). Methods We gave subthreshold electrical stimulation to the left stellate ganglion (LSG) in six normal dogs for 41 ± 9 days (protocol 1) and to six dogs with MI and CAVB for 41 ± 29 days, while continuously monitoring their cardiac rhythm (protocol 2). We also monitored the rhythm of two dogs with MI, CAVB, and NGF infusion to the LSG and determined the ventricular nerve density in six healthy control dogs. Results In protocol 1, the hearts from dogs with LSG electrical stimulation had a higher density of nerve fibers immunopositive to tyrosine hydroxylase, synaptophysin, and growth-associated protein-43 than those of normal control dogs (p < 0.01). In protocol 2, there was a high magnitude of cardiac nerve sprouting in all dogs studied. Ventricular tachycardia ≥8 beats and ≥20 beats was more frequent in dogs with electrical stimulation than in dogs with NGF infusion to the LSG (36 ± 60 and 11 ± 17 vs. 4.7 ± 6.1 and 0.1 ± 0.33 episodes per day, p < 0.05 and p < 0.03, respectively). Four of six dogs in protocol 2 had SCD. Conclusions Subthreshold electrical stimulation of the LSG induces cardiac nerve sprouting and sympathetic hyperinnervation and facilitates the development of a high-yield canine model of ventricular arrhythmia and SCD.

Original languageEnglish (US)
Pages (from-to)858-864
Number of pages7
JournalJournal of the American College of Cardiology
Volume43
Issue number5
DOIs
StatePublished - Mar 3 2004

Fingerprint

Stellate Ganglion
Sudden Cardiac Death
Electric Stimulation
Canidae
Dogs
Atrioventricular Block
Nerve Growth Factor
Myocardial Infarction
GAP-43 Protein
Synaptophysin
Tyrosine 3-Monooxygenase
Ventricular Tachycardia
Nerve Fibers
Cardiac Arrhythmias

Keywords

  • CAVB
  • Complete atrioventricular block
  • DSI
  • Data Sciences International
  • GAP43
  • Growth-associated protein-43
  • ICD
  • Implantable cardioverter-defibrillator
  • LSG
  • LTP
  • Left stellate ganglion
  • Long-term potentiation
  • MI
  • Myocardial infarction
  • NGF
  • Nerve growth factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Long-term subthreshold electrical stimulation of the left stellate ganglion and a canine model of sudden cardiac death. / Swissa, Moshe; Zhou, Shengmei; Gonzalez-Gomez, Ignacio; Chang, Che Ming; Lai, Angela C.; Cates, Adam W.; Fishbein, Michael C.; Karagueuzian, Hrayr S.; Chen, Peng Sheng; Chen, Lan S.

In: Journal of the American College of Cardiology, Vol. 43, No. 5, 03.03.2004, p. 858-864.

Research output: Contribution to journalArticle

Swissa, Moshe ; Zhou, Shengmei ; Gonzalez-Gomez, Ignacio ; Chang, Che Ming ; Lai, Angela C. ; Cates, Adam W. ; Fishbein, Michael C. ; Karagueuzian, Hrayr S. ; Chen, Peng Sheng ; Chen, Lan S. / Long-term subthreshold electrical stimulation of the left stellate ganglion and a canine model of sudden cardiac death. In: Journal of the American College of Cardiology. 2004 ; Vol. 43, No. 5. pp. 858-864.
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abstract = "Objectives We sought to develop a high-yield canine model of sudden cardiac death (SCD). Background Because electrical stimulation is a powerful means to elicit nerve sprouting, we hypothesize that subthreshold electrical stimulation is more effective than nerve growth factor (NGF) infusion in inducing nerve sprouting and SCD in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). Methods We gave subthreshold electrical stimulation to the left stellate ganglion (LSG) in six normal dogs for 41 ± 9 days (protocol 1) and to six dogs with MI and CAVB for 41 ± 29 days, while continuously monitoring their cardiac rhythm (protocol 2). We also monitored the rhythm of two dogs with MI, CAVB, and NGF infusion to the LSG and determined the ventricular nerve density in six healthy control dogs. Results In protocol 1, the hearts from dogs with LSG electrical stimulation had a higher density of nerve fibers immunopositive to tyrosine hydroxylase, synaptophysin, and growth-associated protein-43 than those of normal control dogs (p < 0.01). In protocol 2, there was a high magnitude of cardiac nerve sprouting in all dogs studied. Ventricular tachycardia ≥8 beats and ≥20 beats was more frequent in dogs with electrical stimulation than in dogs with NGF infusion to the LSG (36 ± 60 and 11 ± 17 vs. 4.7 ± 6.1 and 0.1 ± 0.33 episodes per day, p < 0.05 and p < 0.03, respectively). Four of six dogs in protocol 2 had SCD. Conclusions Subthreshold electrical stimulation of the LSG induces cardiac nerve sprouting and sympathetic hyperinnervation and facilitates the development of a high-yield canine model of ventricular arrhythmia and SCD.",
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author = "Moshe Swissa and Shengmei Zhou and Ignacio Gonzalez-Gomez and Chang, {Che Ming} and Lai, {Angela C.} and Cates, {Adam W.} and Fishbein, {Michael C.} and Karagueuzian, {Hrayr S.} and Chen, {Peng Sheng} and Chen, {Lan S.}",
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T1 - Long-term subthreshold electrical stimulation of the left stellate ganglion and a canine model of sudden cardiac death

AU - Swissa, Moshe

AU - Zhou, Shengmei

AU - Gonzalez-Gomez, Ignacio

AU - Chang, Che Ming

AU - Lai, Angela C.

AU - Cates, Adam W.

AU - Fishbein, Michael C.

AU - Karagueuzian, Hrayr S.

AU - Chen, Peng Sheng

AU - Chen, Lan S.

PY - 2004/3/3

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N2 - Objectives We sought to develop a high-yield canine model of sudden cardiac death (SCD). Background Because electrical stimulation is a powerful means to elicit nerve sprouting, we hypothesize that subthreshold electrical stimulation is more effective than nerve growth factor (NGF) infusion in inducing nerve sprouting and SCD in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). Methods We gave subthreshold electrical stimulation to the left stellate ganglion (LSG) in six normal dogs for 41 ± 9 days (protocol 1) and to six dogs with MI and CAVB for 41 ± 29 days, while continuously monitoring their cardiac rhythm (protocol 2). We also monitored the rhythm of two dogs with MI, CAVB, and NGF infusion to the LSG and determined the ventricular nerve density in six healthy control dogs. Results In protocol 1, the hearts from dogs with LSG electrical stimulation had a higher density of nerve fibers immunopositive to tyrosine hydroxylase, synaptophysin, and growth-associated protein-43 than those of normal control dogs (p < 0.01). In protocol 2, there was a high magnitude of cardiac nerve sprouting in all dogs studied. Ventricular tachycardia ≥8 beats and ≥20 beats was more frequent in dogs with electrical stimulation than in dogs with NGF infusion to the LSG (36 ± 60 and 11 ± 17 vs. 4.7 ± 6.1 and 0.1 ± 0.33 episodes per day, p < 0.05 and p < 0.03, respectively). Four of six dogs in protocol 2 had SCD. Conclusions Subthreshold electrical stimulation of the LSG induces cardiac nerve sprouting and sympathetic hyperinnervation and facilitates the development of a high-yield canine model of ventricular arrhythmia and SCD.

AB - Objectives We sought to develop a high-yield canine model of sudden cardiac death (SCD). Background Because electrical stimulation is a powerful means to elicit nerve sprouting, we hypothesize that subthreshold electrical stimulation is more effective than nerve growth factor (NGF) infusion in inducing nerve sprouting and SCD in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). Methods We gave subthreshold electrical stimulation to the left stellate ganglion (LSG) in six normal dogs for 41 ± 9 days (protocol 1) and to six dogs with MI and CAVB for 41 ± 29 days, while continuously monitoring their cardiac rhythm (protocol 2). We also monitored the rhythm of two dogs with MI, CAVB, and NGF infusion to the LSG and determined the ventricular nerve density in six healthy control dogs. Results In protocol 1, the hearts from dogs with LSG electrical stimulation had a higher density of nerve fibers immunopositive to tyrosine hydroxylase, synaptophysin, and growth-associated protein-43 than those of normal control dogs (p < 0.01). In protocol 2, there was a high magnitude of cardiac nerve sprouting in all dogs studied. Ventricular tachycardia ≥8 beats and ≥20 beats was more frequent in dogs with electrical stimulation than in dogs with NGF infusion to the LSG (36 ± 60 and 11 ± 17 vs. 4.7 ± 6.1 and 0.1 ± 0.33 episodes per day, p < 0.05 and p < 0.03, respectively). Four of six dogs in protocol 2 had SCD. Conclusions Subthreshold electrical stimulation of the LSG induces cardiac nerve sprouting and sympathetic hyperinnervation and facilitates the development of a high-yield canine model of ventricular arrhythmia and SCD.

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KW - Complete atrioventricular block

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KW - Data Sciences International

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KW - Growth-associated protein-43

KW - ICD

KW - Implantable cardioverter-defibrillator

KW - LSG

KW - LTP

KW - Left stellate ganglion

KW - Long-term potentiation

KW - MI

KW - Myocardial infarction

KW - NGF

KW - Nerve growth factor

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