Loss of BRM expression is a frequently observed event in poorly differentiated clear cell renal cell carcinoma

Qiu yuan Xia, Qiu Rao, Liang Cheng, Qin Shen, Shan shan Shi, Li Li, Biao Liu, Jin Zhang, Yan fen Wang, Qun li Shi, Jian dong Wang, Heng hui Ma, Zhen feng Lu, Bo Yu, Ru song Zhang, Xiao jun Zhou

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Aims: The aim of this study was to examine the status of Brahma (BRM), a key SWI/SNF complex subunit, in clear cell renal cell carcinomas (RCCs), and to analyse the histopathology, immunophenotype, molecular features and prognosis of the BRM-negative cases. Methods and results: We identified 19 cases of grade 4 tumours lacking BRM expression among 625 clear cell RCCs. All 19 cases exhibited features of poor differentiation: 13 showed pure poorly differentiated morphology, while six were composite tumours with an admixed typical low-grade component. Besides negative BRM expression, the immunophenotype of these cases was similar to clear cell RCC. VHL gene mutations were identified in nine of the 19 patients (47%). Chromosome 3p deletion was detected in 11 of 13 poorly differentiated RCCs and both areas of five of five composite tumours. All poorly differentiated tumour areas showed polysomy of chromosome 3. No losses or gains of chromosome 3 were observed in low-grade tumour areas of five of five composite RCCs. Conclusions: We have shown that loss of BRM expression is a common feature among poorly differentiated tumours in clear cell RCCs. We hypothesize that loss of BRM expression is involved in tumor de-differentiation in clear cell RCCs and may play an important role during tumour progression.

Original languageEnglish (US)
Pages (from-to)847-862
Number of pages16
JournalHistopathology
Volume64
Issue number6
DOIs
StatePublished - May 2014

Keywords

  • BRM/SMARCA2
  • Clear cell carcinoma
  • Fluorescence in-situ hybridization
  • High-grade
  • Immunohistochemistry
  • Poorly differentiated
  • Renal cell carcinoma
  • Rhabdoid
  • SWI/SNF complex
  • Von Hippel-Lindau

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Medicine(all)

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  • Cite this

    Xia, Q. Y., Rao, Q., Cheng, L., Shen, Q., Shi, S. S., Li, L., Liu, B., Zhang, J., Wang, Y. F., Shi, Q. L., Wang, J. D., Ma, H. H., Lu, Z. F., Yu, B., Zhang, R. S., & Zhou, X. J. (2014). Loss of BRM expression is a frequently observed event in poorly differentiated clear cell renal cell carcinoma. Histopathology, 64(6), 847-862. https://doi.org/10.1111/his.12334