Loss of calcium/calmodulin-dependent protein kinase II activity in cortical astrocytes decreases glutamate uptake and induces neurotoxic release of ATP

Nicole M. Ashpole, Aarti R. Chawla, Matthew P. Martin, Tatiana Brustovetskys, Nickolay Brustovetskys, Andy Hudmon

Research output: Contribution to journalArticle

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Abstract

The extent of calcium/calmodulin-dependent protein kinase II (CaMKII) inactivation in the brain after ischemia correlates with the extent of damage. We have previously shown that a loss of CaMKII activity in neurons is detrimental to neuronal viability by inducing excitotoxic glutamate release. In the current study we extend these findings to show that the ability of astrocytes to buffer extracellular glutamate is reduced when CaMKII is inhibited. Furthermore, CaMKII inhibition in astrocytes is associated with the rapid onset of intracellular calcium oscillations. Surprisingly, this rapid calcium influx is blocked by the N-type calcium channel antagonist, ω-conotoxin. Although the function of N-type calcium channels within astrocytes is controversial, these voltage-gated calcium channels have been linked to calcium-dependent vesicular gliotransmitter release. When extracellular glutamate and ATP levels are measured after CaMKII inhibition within our enriched astrocyte cultures, no alterations in glutamate levels are observed, whereas ATP levels in the extracellular environment significantly increase. Extracellular ATP accumulation associated with CaMKII inhibition contributes both to calcium oscillations within astrocytes and ultimately cortical neuron toxicity. Thus, a loss of CaMKII signaling within astrocytes dysregulates glutamate uptake and supports ATP release, two processes that would compromise neuronal survival after ischemic/excitotoxic insults.

Original languageEnglish (US)
Pages (from-to)14599-14611
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number20
DOIs
StatePublished - May 17 2013

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Astrocytes
Glutamic Acid
Adenosine Triphosphate
N-Type Calcium Channels
Calcium
Calcium Signaling
Neurons
Conotoxins
Calcium Channel Blockers
Calcium Channels
Brain Ischemia
Toxicity
Brain
Buffers
Electric potential

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Loss of calcium/calmodulin-dependent protein kinase II activity in cortical astrocytes decreases glutamate uptake and induces neurotoxic release of ATP. / Ashpole, Nicole M.; Chawla, Aarti R.; Martin, Matthew P.; Brustovetskys, Tatiana; Brustovetskys, Nickolay; Hudmon, Andy.

In: Journal of Biological Chemistry, Vol. 288, No. 20, 17.05.2013, p. 14599-14611.

Research output: Contribution to journalArticle

Ashpole, Nicole M. ; Chawla, Aarti R. ; Martin, Matthew P. ; Brustovetskys, Tatiana ; Brustovetskys, Nickolay ; Hudmon, Andy. / Loss of calcium/calmodulin-dependent protein kinase II activity in cortical astrocytes decreases glutamate uptake and induces neurotoxic release of ATP. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 20. pp. 14599-14611.
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