Loss of corneal sensory nerve fibers in SIV-infected macaques

An alternate approach to investigate HIV-induced PNS damage

Jamie L. Dorsey, Lisa M. Mangus, Jonathan D. Oakley, Sarah E. Beck, Kathleen M. Kelly, Suzanne E. Queen, Kelly A. Metcalf Pate, Robert J. Adams, Carl Marfurt, Joseph L. Mankowski

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peripheral neuropathy is the most frequent neurological complication of HIV infection, affecting more than one-third of infected patients, including patients treated with antiretroviral therapy. Although emerging noninvasive techniques for corneal nerve assessments are increasingly being used to diagnose and monitor peripheral neuropathies, corneal nerve alterations have not been characterized in HIV. Here, to determine whether SIV infection leads to corneal nerve fiber loss, we immunostained corneas for the nerve fiber marker βIII tubulin. We developed and applied both manual and automated methods to measure nerves in the corneal subbasal plexus. These counting methods independently indicated significantly lower subbasal corneal nerve fiber density among SIV-infected animals that rapidly progressed to AIDS compared with slow progressors. Concomitant with decreased corneal nerve fiber density, rapid progressors had increased levels of SIV RNA and CD68-positive macrophages and expression of glial fibrillary acidic protein by glial satellite cells in the trigeminal ganglia, the location of the neuronal cell bodies of corneal sensory nerve fibers. In addition, corneal nerve fiber density was directly correlated with epidermal nerve fiber length. These findings indicate that corneal nerve assessment has great potential to diagnose and monitor HIV-induced peripheral neuropathy and to set the stage for introducing noninvasive techniques to measure corneal nerve fiber density in HIV clinical settings.

Original languageEnglish
Pages (from-to)1652-1659
Number of pages8
JournalAmerican Journal of Pathology
Volume184
Issue number6
DOIs
StatePublished - 2014

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Macaca
Nerve Fibers
HIV
Peripheral Nervous System Diseases
Trigeminal Ganglion
Glial Fibrillary Acidic Protein
Tubulin
Neuroglia
Cornea
HIV Infections
Acquired Immunodeficiency Syndrome
Macrophages
RNA
Infection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Dorsey, J. L., Mangus, L. M., Oakley, J. D., Beck, S. E., Kelly, K. M., Queen, S. E., ... Mankowski, J. L. (2014). Loss of corneal sensory nerve fibers in SIV-infected macaques: An alternate approach to investigate HIV-induced PNS damage. American Journal of Pathology, 184(6), 1652-1659. https://doi.org/10.1016/j.ajpath.2014.02.009

Loss of corneal sensory nerve fibers in SIV-infected macaques : An alternate approach to investigate HIV-induced PNS damage. / Dorsey, Jamie L.; Mangus, Lisa M.; Oakley, Jonathan D.; Beck, Sarah E.; Kelly, Kathleen M.; Queen, Suzanne E.; Metcalf Pate, Kelly A.; Adams, Robert J.; Marfurt, Carl; Mankowski, Joseph L.

In: American Journal of Pathology, Vol. 184, No. 6, 2014, p. 1652-1659.

Research output: Contribution to journalArticle

Dorsey, JL, Mangus, LM, Oakley, JD, Beck, SE, Kelly, KM, Queen, SE, Metcalf Pate, KA, Adams, RJ, Marfurt, C & Mankowski, JL 2014, 'Loss of corneal sensory nerve fibers in SIV-infected macaques: An alternate approach to investigate HIV-induced PNS damage', American Journal of Pathology, vol. 184, no. 6, pp. 1652-1659. https://doi.org/10.1016/j.ajpath.2014.02.009
Dorsey, Jamie L. ; Mangus, Lisa M. ; Oakley, Jonathan D. ; Beck, Sarah E. ; Kelly, Kathleen M. ; Queen, Suzanne E. ; Metcalf Pate, Kelly A. ; Adams, Robert J. ; Marfurt, Carl ; Mankowski, Joseph L. / Loss of corneal sensory nerve fibers in SIV-infected macaques : An alternate approach to investigate HIV-induced PNS damage. In: American Journal of Pathology. 2014 ; Vol. 184, No. 6. pp. 1652-1659.
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