Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic db/db mice

Eleni Beli, Samantha Prabakaran, Preethi Krishnan, Carmella Evans-Molina, Maria B. Grant

Research output: Contribution to journalArticle

Abstract

Our hypothesis is that diabetes leads to loss of diurnal oscillatory rhythms in gut microbiota altering circulating metabolites. We performed an observational study where we compared diurnal changes of the gut microbiota with temporal changes of plasma metabolites. Metadata analysis from bacterial DNA from fecal pellets collected from 10-month old control (db/m) and type 2 diabetic (db/db) mice every 4 h for a 24-h period was used for prediction analysis. Blood plasma was collected at a day and night time points and was used for untargeted global metabolomic analysis. Feeding and activity behaviors were recorded. Our results show that while diabetic mice exhibited feeding and activity behavior similar to control mice, they exhibited a loss of diurnal oscillations in bacteria of the genus Akkermansia, Bifidobacterium, Allobaculum, Oscillospira and a phase shift in the oscillations of g.Prevotella, proteobacteria, and actinobacteria. Analysis of the circulating metabolites showed alterations in the diurnal pattern of metabolic pathways where bacteria have been implicated, such as the histidine, betaine, and methionine/cysteine pathway, mitochondrial function and the urea cycle. Functional analysis of the differential microbes revealed that during the day, when mice are asleep, the microbes of diabetic mice were enriched in processing carbon and pyruvate metabolic pathways instead of xenobiotic degradation as was observed for control mice. Altogether, our study suggests that diabetes led to loss of rhythmic oscillations of many gut microbiota with possible implications for temporal regulation of host metabolic pathways.

Original languageEnglish (US)
Article number2310
JournalNutrients
Volume11
Issue number10
DOIs
StatePublished - Oct 2019

Fingerprint

Circadian Rhythm
intestinal microorganisms
circadian rhythm
metabolites
mice
Metabolic Networks and Pathways
biochemical pathways
oscillation
Feeding Behavior
Oscillospira
diabetes
Prevotella
Bacteria
microorganisms
Proteobacteria
Betaine
Bacterial DNA
Bifidobacterium
Metabolomics
Actinobacteria

Keywords

  • Circadian
  • Histidine
  • Metabolites
  • Methionine/cysteine
  • Microbiota
  • TCA cycle
  • TMAO
  • Type 2 diabetes
  • Urea cycle

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Loss of diurnal oscillatory rhythms in gut microbiota correlates with changes in circulating metabolites in type 2 diabetic db/db mice. / Beli, Eleni; Prabakaran, Samantha; Krishnan, Preethi; Evans-Molina, Carmella; Grant, Maria B.

In: Nutrients, Vol. 11, No. 10, 2310, 10.2019.

Research output: Contribution to journalArticle

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