Loss of growth hormone-dependent characteristics of rat hepatocytes in culture

David Crabb, Janet Roepke

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The liver of rodents is sexually differentiated, i.e. the female liver differs from the male liver. This differentiation is largely controlled by the pattern of growth hormone (GH) secretion. We have attempted to maintain GH-dependent differentiation of cultured rat hepatocytes. We examined the level of alcohol dehydrogenase (ADH) activity, which responds to GH and is higher in female than in male liver, and the estrogen receptor, which is dependent on GH but is present in equal amounts in males and females. ADH activity was maintained in cells from male rats, but fell by 40% in cells from females in medium supplemented with insulin and dexamethasone. The estrogen receptor content of female cells fell dramatically to undetectable levels within 2 d of culture. Extensive supplementation of the medium failed to prevent the decrease in ADH activity in female cells; similarly, the addition of female sex steroids; rat serum; pituitary extracts; rat, human, or bovine GH; or ovine prolactin failed to maintain the enzyme activity. Insulin, dexamethasone, thyroid hormone plus GH or prolactin, or the combination of all five hormones also failed to prevent the loss of estrogen receptors. Short-term cultures of rat hepatocytes, although retaining the liver-specific expression of ADH at the male level, lose GH-dependent expression of the estrogen receptor and stimulation of ADH activity.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume23
Issue number4
DOIs
StatePublished - Apr 1987

Fingerprint

Alcohol Dehydrogenase
Growth Hormone
Rats
Hepatocytes
Liver
Estrogen Receptors
Prolactin
Dexamethasone
Insulin
Human Growth Hormone
Enzyme activity
Thyroid Hormones
Steroids
Rodentia
Sheep
Hormones
Enzymes
Serum

Keywords

  • alcohol dehydrogenase
  • cultured hepatocytes
  • estrogen receptor
  • growth hormone

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Medicine(all)

Cite this

Loss of growth hormone-dependent characteristics of rat hepatocytes in culture. / Crabb, David; Roepke, Janet.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 23, No. 4, 04.1987, p. 303-307.

Research output: Contribution to journalArticle

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