Loss of heterozygosity analysis to define putative region involved in tumor differentiation and metastases in sporadic colorectal cancer patients

Zhihai Peng, Fang Zhang, Chongzhi Zhou, Guoqiang Qiu, Shaochun Bai, Wanqing Liu, Lin He

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: To detect putative suppressor loci involved in tumor progressing or metastases. METHODS: Thirty microsatellite marker primers were employed to amply the corresponding loci of the genome DNA from 83 patients with sporadic colorectal cancer. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed by Genotyper and Genescan software. RESULTS: The data were obtained from 24 loci, with an average LOH frequency of 15.16%. The LOH at D2S206 and D2S364 was more frequent than 30%, and was less than 20% at the rest loci. Significant difference was observed between the percentage of LOH and tumor staging or differentiation at D2S142 (2q24.1), D2S126 (2q35), D2S2211 (2p24.2), D2S305 (2p23.3). Occarrence of deletion at the later two loci was correlative. CONCLUSIONS: Frequent LOH was not observed at the loci around known mismatch repair genes on chr. 2. The region between D2S305 (2p23.3) and D2S2211 (2p24.2) deleted holistically, and was correlated to the stage and differentiation of tumor attended by D2S142 (2q24.1) and D2S126 (2q35) on 2q. It is suggested that unknown genes associated with tumor progressing or metastases reside in the two loci on 2q or the region on 2p.

Original languageEnglish (US)
Pages (from-to)776-779
Number of pages4
JournalZhonghua wai ke za zhi [Chinese journal of surgery]
Volume40
Issue number10
StatePublished - 2002
Externally publishedYes

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Loss of Heterozygosity
Colorectal Neoplasms
Neoplasm Metastasis
Neoplasms
DNA Mismatch Repair
Neoplasm Staging
Microsatellite Repeats
Genes
Software
Genome
Polymerase Chain Reaction
DNA

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Loss of heterozygosity analysis to define putative region involved in tumor differentiation and metastases in sporadic colorectal cancer patients. / Peng, Zhihai; Zhang, Fang; Zhou, Chongzhi; Qiu, Guoqiang; Bai, Shaochun; Liu, Wanqing; He, Lin.

In: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 40, No. 10, 2002, p. 776-779.

Research output: Contribution to journalArticle

Peng, Zhihai ; Zhang, Fang ; Zhou, Chongzhi ; Qiu, Guoqiang ; Bai, Shaochun ; Liu, Wanqing ; He, Lin. / Loss of heterozygosity analysis to define putative region involved in tumor differentiation and metastases in sporadic colorectal cancer patients. In: Zhonghua wai ke za zhi [Chinese journal of surgery]. 2002 ; Vol. 40, No. 10. pp. 776-779.
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abstract = "OBJECTIVE: To detect putative suppressor loci involved in tumor progressing or metastases. METHODS: Thirty microsatellite marker primers were employed to amply the corresponding loci of the genome DNA from 83 patients with sporadic colorectal cancer. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed by Genotyper and Genescan software. RESULTS: The data were obtained from 24 loci, with an average LOH frequency of 15.16{\%}. The LOH at D2S206 and D2S364 was more frequent than 30{\%}, and was less than 20{\%} at the rest loci. Significant difference was observed between the percentage of LOH and tumor staging or differentiation at D2S142 (2q24.1), D2S126 (2q35), D2S2211 (2p24.2), D2S305 (2p23.3). Occarrence of deletion at the later two loci was correlative. CONCLUSIONS: Frequent LOH was not observed at the loci around known mismatch repair genes on chr. 2. The region between D2S305 (2p23.3) and D2S2211 (2p24.2) deleted holistically, and was correlated to the stage and differentiation of tumor attended by D2S142 (2q24.1) and D2S126 (2q35) on 2q. It is suggested that unknown genes associated with tumor progressing or metastases reside in the two loci on 2q or the region on 2p.",
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T1 - Loss of heterozygosity analysis to define putative region involved in tumor differentiation and metastases in sporadic colorectal cancer patients

AU - Peng, Zhihai

AU - Zhang, Fang

AU - Zhou, Chongzhi

AU - Qiu, Guoqiang

AU - Bai, Shaochun

AU - Liu, Wanqing

AU - He, Lin

PY - 2002

Y1 - 2002

N2 - OBJECTIVE: To detect putative suppressor loci involved in tumor progressing or metastases. METHODS: Thirty microsatellite marker primers were employed to amply the corresponding loci of the genome DNA from 83 patients with sporadic colorectal cancer. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed by Genotyper and Genescan software. RESULTS: The data were obtained from 24 loci, with an average LOH frequency of 15.16%. The LOH at D2S206 and D2S364 was more frequent than 30%, and was less than 20% at the rest loci. Significant difference was observed between the percentage of LOH and tumor staging or differentiation at D2S142 (2q24.1), D2S126 (2q35), D2S2211 (2p24.2), D2S305 (2p23.3). Occarrence of deletion at the later two loci was correlative. CONCLUSIONS: Frequent LOH was not observed at the loci around known mismatch repair genes on chr. 2. The region between D2S305 (2p23.3) and D2S2211 (2p24.2) deleted holistically, and was correlated to the stage and differentiation of tumor attended by D2S142 (2q24.1) and D2S126 (2q35) on 2q. It is suggested that unknown genes associated with tumor progressing or metastases reside in the two loci on 2q or the region on 2p.

AB - OBJECTIVE: To detect putative suppressor loci involved in tumor progressing or metastases. METHODS: Thirty microsatellite marker primers were employed to amply the corresponding loci of the genome DNA from 83 patients with sporadic colorectal cancer. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed by Genotyper and Genescan software. RESULTS: The data were obtained from 24 loci, with an average LOH frequency of 15.16%. The LOH at D2S206 and D2S364 was more frequent than 30%, and was less than 20% at the rest loci. Significant difference was observed between the percentage of LOH and tumor staging or differentiation at D2S142 (2q24.1), D2S126 (2q35), D2S2211 (2p24.2), D2S305 (2p23.3). Occarrence of deletion at the later two loci was correlative. CONCLUSIONS: Frequent LOH was not observed at the loci around known mismatch repair genes on chr. 2. The region between D2S305 (2p23.3) and D2S2211 (2p24.2) deleted holistically, and was correlated to the stage and differentiation of tumor attended by D2S142 (2q24.1) and D2S126 (2q35) on 2q. It is suggested that unknown genes associated with tumor progressing or metastases reside in the two loci on 2q or the region on 2p.

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