Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption

Michael S. Eaton, Nicholas Weinstein, Jordan B. Newby, Maggie M. Plattes, Hanna E. Foster, Jon W. Arthur, Taylor D. Ward, Stephen R. Shively, Ryann Shor, Justin Nathan, Hannah M. Davis, Lilian Plotkin, Eric M. Wauson, Brian J. Dewar, Aaron Broege, Jonathan W. Lowery

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The taste receptor type 1 (TAS1R) family of heterotrimeric G protein-coupled receptors participates in monitoring energy and nutrient status. TAS1R member 3 (TAS1R3) is a bi-functional protein that recognizes amino acids such as L-glycine and L-glutamate or sweet molecules such as sucrose and fructose when dimerized with TAS1R member 1 (TAS1R1) or TAS1R member 2 (TAS1R2), respectively. It was recently reported that deletion of TAS1R3 expression in Tas1R3 mutant mice leads to increased cortical bone mass but the underlying cellular mechanism leading to this phenotype remains unclear. Here, we independently corroborate the increased thickness of cortical bone in femurs of 20-week-old male Tas1R3 mutant mice and confirm that Tas1R3 is expressed in the bone environment. Tas1R3 is expressed in undifferentiated bone marrow stromal cells (BMSCs) in vitro and its expression is maintained during BMP2-induced osteogenic differentiation. However, levels of the bone formation marker procollagen type I N-terminal propeptide (PINP) are unchanged in the serum of 20-week-old Tas1R3 mutant mice as compared to controls. In contrast, levels of the bone resorption marker collagen type I C-telopeptide are reduced greater than 60% in Tas1R3 mutant mice. Consistent with this, Tas1R3 and its putative signaling partner Tas1R2 are expressed in primary osteoclasts and their expression levels positively correlate with differentiation status. Collectively, these findings suggest that high bone mass in Tas1R3 mutant mice is due to uncoupled bone remodeling with reduced osteoclast function and provide rationale for future experiments examining the cell-type-dependent role for TAS1R family members in nutrient sensing in postnatal bone remodeling.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Physiology and Biochemistry
DOIs
StateAccepted/In press - Oct 10 2017

Fingerprint

Bone Resorption
Nutrients
Bone
Food
Sensors
Bone Remodeling
Osteoclasts
Collagen Type I
Heterotrimeric GTP-Binding Proteins
Bone and Bones
G-Protein-Coupled Receptors
Fructose
Mesenchymal Stromal Cells
Osteogenesis
Femur
Glycine
Sucrose
Glutamic Acid
Phenotype
Amino Acids

Keywords

  • Bone
  • Osteoblast
  • Osteoclast
  • TAS1R1
  • TAS1R2
  • TAS1R3
  • Taste receptor

ASJC Scopus subject areas

  • Physiology
  • Biochemistry

Cite this

Eaton, M. S., Weinstein, N., Newby, J. B., Plattes, M. M., Foster, H. E., Arthur, J. W., ... Lowery, J. W. (Accepted/In press). Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption. Journal of Physiology and Biochemistry, 1-6. https://doi.org/10.1007/s13105-017-0596-7

Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption. / Eaton, Michael S.; Weinstein, Nicholas; Newby, Jordan B.; Plattes, Maggie M.; Foster, Hanna E.; Arthur, Jon W.; Ward, Taylor D.; Shively, Stephen R.; Shor, Ryann; Nathan, Justin; Davis, Hannah M.; Plotkin, Lilian; Wauson, Eric M.; Dewar, Brian J.; Broege, Aaron; Lowery, Jonathan W.

In: Journal of Physiology and Biochemistry, 10.10.2017, p. 1-6.

Research output: Contribution to journalArticle

Eaton, MS, Weinstein, N, Newby, JB, Plattes, MM, Foster, HE, Arthur, JW, Ward, TD, Shively, SR, Shor, R, Nathan, J, Davis, HM, Plotkin, L, Wauson, EM, Dewar, BJ, Broege, A & Lowery, JW 2017, 'Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption', Journal of Physiology and Biochemistry, pp. 1-6. https://doi.org/10.1007/s13105-017-0596-7
Eaton, Michael S. ; Weinstein, Nicholas ; Newby, Jordan B. ; Plattes, Maggie M. ; Foster, Hanna E. ; Arthur, Jon W. ; Ward, Taylor D. ; Shively, Stephen R. ; Shor, Ryann ; Nathan, Justin ; Davis, Hannah M. ; Plotkin, Lilian ; Wauson, Eric M. ; Dewar, Brian J. ; Broege, Aaron ; Lowery, Jonathan W. / Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption. In: Journal of Physiology and Biochemistry. 2017 ; pp. 1-6.
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AU - Foster, Hanna E.

AU - Arthur, Jon W.

AU - Ward, Taylor D.

AU - Shively, Stephen R.

AU - Shor, Ryann

AU - Nathan, Justin

AU - Davis, Hannah M.

AU - Plotkin, Lilian

AU - Wauson, Eric M.

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