Loss of urinary macromolecules in mice causes interstitial and intratubular renal calcification dependent on the underlying conditions

Xue Ru Wu, John C. Lieske, Andrew P. Evan, Andre J. Sommer, Lucy Liaw, Lan Mo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Urinary protein macromolecules have long been thought to play a role in influencing the various phases of urolithiasis including nucleation, growth, aggregation of mineral crystals and their subsequent adhesion to the renal epithelial cells. However, compelling evidence regarding their precise role was lacking, due partly to the fact that most prior studies were done in vitro and results were highly variable depending on the experimental conditions. The advent of genetic engineering technology has made it possible to study urinary protein macromolecules within an in vivo biological system. Indeed, recent studies have begun to shed light on the net effects of loss of one or more macromolecules on the earliest steps of urolithiasis. This paper focuses on the in vivo consequences of inactivating Tamm-Horsfall protein and/or osteopontin, two major urinary glycoproteins, using the knockout approach. The renal phenotypes of both single and double knockout mice under spontaneous or hyperoxaluric conditions will be described. The functional significance of the urinary macromolecules as critical defense factors against renal calcification will also be discussed.

Original languageEnglish (US)
Title of host publicationRenal Stone Disease 2 - 2nd International Urolithiasis Research Symposium
Pages113-119
Number of pages7
DOIs
StatePublished - Oct 22 2008
Event2nd International Urolithiasis Research Symposium - Indianapolis, IN, United States
Duration: Apr 17 2008Apr 18 2008

Publication series

NameAIP Conference Proceedings
Volume1049
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other2nd International Urolithiasis Research Symposium
CountryUnited States
CityIndianapolis, IN
Period4/17/084/18/08

Keywords

  • Kidney stone
  • Knockout mice
  • Osteopontin
  • Tamm-Horsfall protein
  • Urinary macromolecules
  • Urolithiasis

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Wu, X. R., Lieske, J. C., Evan, A. P., Sommer, A. J., Liaw, L., & Mo, L. (2008). Loss of urinary macromolecules in mice causes interstitial and intratubular renal calcification dependent on the underlying conditions. In Renal Stone Disease 2 - 2nd International Urolithiasis Research Symposium (pp. 113-119). (AIP Conference Proceedings; Vol. 1049). https://doi.org/10.1063/1.2998004