Losses of 3p, 11p, and 13q in EJ/ras-transformable Simian virus 40- immortalized human uroepithelial cells

Chinghai Kao, S. Q. Wu, M. Bhatthacharya, L. F. Meisner, C. A. Reznikoff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Five independent clones of Simian virus 40 (SV40)-immortalized human uroepithelial cells (CK/SV-HUC) were established after transfection of HUC cultures from the same tissue donor with plasmids encoding SV40 large T and small t antigen genes. Each CK/SV-HUC clone contained a unique SV40 integration site, and all expressed similar levels of SV40 mRNA. All five clones were nontumorigenic, but clones 2, 4, and 5 tumorigenically transformed after transfection at P19 with mutant EJ/ras and also spontaneously after 40 serial passages in vitro. In contrast, CK/SV-HUC clones 1 and 3 did not transform when either approach was used. These differences in transformability among CK/SV-HUC clones could not be predicted based on differences in SV40 gene expression nor on any in vitro growth property tested. In cytogenetic analyses, a transformable clone showed losses of three chromosome arms containing putative cancer suppressor gene regions, including 3p14→pter, 13q, and 11p, whereas the nontransformable clones showed none of these losses. Thus these data indicate that genetic losses on 3p, 11p, and 13q may contribute to tumorigenic transformation of SV40- immortalized human uroepithelial cells.

Original languageEnglish (US)
Pages (from-to)158-168
Number of pages11
JournalGenes Chromosomes and Cancer
Volume4
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

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Simian virus 40
Clone Cells
Transfection
Neoplastic Cell Transformation
Virus Integration
Serial Passage
Cytogenetic Analysis
Viral Tumor Antigens
Tumor Suppressor Genes
Plasmids
Chromosomes
Tissue Donors
Gene Expression
Messenger RNA
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Losses of 3p, 11p, and 13q in EJ/ras-transformable Simian virus 40- immortalized human uroepithelial cells. / Kao, Chinghai; Wu, S. Q.; Bhatthacharya, M.; Meisner, L. F.; Reznikoff, C. A.

In: Genes Chromosomes and Cancer, Vol. 4, No. 2, 1992, p. 158-168.

Research output: Contribution to journalArticle

Kao, Chinghai ; Wu, S. Q. ; Bhatthacharya, M. ; Meisner, L. F. ; Reznikoff, C. A. / Losses of 3p, 11p, and 13q in EJ/ras-transformable Simian virus 40- immortalized human uroepithelial cells. In: Genes Chromosomes and Cancer. 1992 ; Vol. 4, No. 2. pp. 158-168.
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