Lovastatin effects of human breast carcinoma cells. Differential toxicity of an adriamycin-resistant derivative and influence on selenocysteine tRNAs

Alan M. Diamond, Deborah Jaffe, Judith L. Murray, Ahmad Safa, Brian L. Samuels, Dolph L. Hatfield

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Selenocysteine tRNA([Ser]Sec) isoacceptors contain the modified nucleotide i6A immediately 3' to the anticodon. Because synthesis of i6A is expected to be inhibited by lovastatin, the status of tRNA([Ser]Sec) isoacceptors was examined in human breast carcinoma cells. As part of the initial characterization of these cells, it was determined that an adriamycin resistant derivative of the MCF-7 cell line exhibited a dramatic increase in the sensitivity to the killing effects of lovastatin relative to the parental MCF-7 cells. When MCF-7(Adr) cells were incubated with high levels of lovastatin, there was a dramatic perturbation in the distribution of isoacceptors within the selenocysteine tRNA population. Lovastatin may therefore be a useful reagent for both the study of differential killing of drug-resistant tumor cells and selenoprotein biosynthesis.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalBiochemistry and Molecular Biology International
Volume38
Issue number2
StatePublished - 1996
Externally publishedYes

Fingerprint

Lovastatin
Doxorubicin
Toxicity
MCF-7 Cells
Cells
Breast Neoplasms
Derivatives
Selenoproteins
Anticodon
Biosynthesis
Transfer RNA
Tumors
Nucleotides
Cell Line
selenocysteinyl-tRNA
Pharmaceutical Preparations
Population
Neoplasms
selenocysteine-tRNA

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Lovastatin effects of human breast carcinoma cells. Differential toxicity of an adriamycin-resistant derivative and influence on selenocysteine tRNAs. / Diamond, Alan M.; Jaffe, Deborah; Murray, Judith L.; Safa, Ahmad; Samuels, Brian L.; Hatfield, Dolph L.

In: Biochemistry and Molecular Biology International, Vol. 38, No. 2, 1996, p. 345-355.

Research output: Contribution to journalArticle

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