Lovastatin effects of human breast carcinoma cells. Differential toxicity of an adriamycin-resistant derivative and influence on selenocysteine tRNAs

Alan M. Diamond, Deborah Jaffe, Judith L. Murray, Ahmad R. Safa, Brian L. Samuels, Dolph L. Hatfield

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Selenocysteine tRNA([Ser]Sec) isoacceptors contain the modified nucleotide i6A immediately 3' to the anticodon. Because synthesis of i6A is expected to be inhibited by lovastatin, the status of tRNA([Ser]Sec) isoacceptors was examined in human breast carcinoma cells. As part of the initial characterization of these cells, it was determined that an adriamycin resistant derivative of the MCF-7 cell line exhibited a dramatic increase in the sensitivity to the killing effects of lovastatin relative to the parental MCF-7 cells. When MCF-7(Adr) cells were incubated with high levels of lovastatin, there was a dramatic perturbation in the distribution of isoacceptors within the selenocysteine tRNA population. Lovastatin may therefore be a useful reagent for both the study of differential killing of drug-resistant tumor cells and selenoprotein biosynthesis.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalBiochemistry and Molecular Biology International
Volume38
Issue number2
StatePublished - Aug 20 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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