Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice

Karen Carver-Moore, Hal Broxmeyer, Shiuh Ming Luoh, Scott Cooper, Jipeng Peng, Samuel A. Burstein, Mark W. Moore, Frederic J. De Sauvage

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Thrombopoietin (TPO), the ligand for the c-mpl receptor, has been shown to be the major regulator of platelet production. Mice deficient in either c- mpl or TPO generated by homologous recombination show a dramatic decrease in platelet counts, but other blood cell counts are normal. Because TPO treatment of myelosuppressed mice not only enhances the recovery of platelets but also accelerates erythroid recovery, we investigated the levels of myeloid and erythroid progenitor cells in TPO- or c-mpl-deficient mice. Our results show that the number of megakaryocyte, granulocyte-macrophage, erythroid, and multilineage progenitors are significantly reduced in the bone marrow, spleen, and peripheral blood of either TPO- or c-mpl-deficient mice. Administration of recombinant murine TPO to TPO-deficient mice and control littermate mice significantly increased the absolute number of myeloid, erythroid, and mixed progenitors in bone marrow and spleen. This increase was especially apparent in TPO-deficient mice where numbers were increased to a level greater than in diluent-treated control mice and approached or equaled that in the TPO-treated control mice. Moreover, TPO-administration greatly increased the number of circulating progenitors as well as platelets in both TPO-deficient and control mice. Furthermore, the megakaryocytopoietic activity of other cytokines in the absence of a functional TPO or c-mpl gene was shown both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)803-808
Number of pages6
JournalBlood
Volume88
Issue number3
StatePublished - Aug 1 1996
Externally publishedYes

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Thrombopoietin
Platelets
Blood Platelets
Bone
Blood
Spleen
Bone Marrow
Myeloid Progenitor Cells
Recovery
Erythroid Precursor Cells
Megakaryocytes
Blood Cell Count
Macrophages
Homologous Recombination
Platelet Count
Granulocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Carver-Moore, K., Broxmeyer, H., Luoh, S. M., Cooper, S., Peng, J., Burstein, S. A., ... De Sauvage, F. J. (1996). Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice. Blood, 88(3), 803-808.

Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice. / Carver-Moore, Karen; Broxmeyer, Hal; Luoh, Shiuh Ming; Cooper, Scott; Peng, Jipeng; Burstein, Samuel A.; Moore, Mark W.; De Sauvage, Frederic J.

In: Blood, Vol. 88, No. 3, 01.08.1996, p. 803-808.

Research output: Contribution to journalArticle

Carver-Moore, K, Broxmeyer, H, Luoh, SM, Cooper, S, Peng, J, Burstein, SA, Moore, MW & De Sauvage, FJ 1996, 'Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice', Blood, vol. 88, no. 3, pp. 803-808.
Carver-Moore K, Broxmeyer H, Luoh SM, Cooper S, Peng J, Burstein SA et al. Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice. Blood. 1996 Aug 1;88(3):803-808.
Carver-Moore, Karen ; Broxmeyer, Hal ; Luoh, Shiuh Ming ; Cooper, Scott ; Peng, Jipeng ; Burstein, Samuel A. ; Moore, Mark W. ; De Sauvage, Frederic J. / Low levels of erythroid and myeloid progenitors in thrombopoietin- and c- mpl-deficient mice. In: Blood. 1996 ; Vol. 88, No. 3. pp. 803-808.
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